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Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water

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Abstract

Introduction

Active microorganisms have been recently discovered in clouds, thus demonstrating the capacity of microorganisms to exist in harsh environments, including exposure to UV and oxidants, osmotic and cold shocks, etc. It is important to understand how microorganisms respond to and survive such stresses at the metabolic level.

Objectives

The objective of this work is to assess metabolome modulation in a strain of Pseudomonas syringae isolated from cloud water and facing temperature downshift from 17 to 5 °C by identifying key molecules and pathways of the response/adaptation to cold shock.

Methods

Bacterial extracts from suspensions of cells grown at 17 °C and further incubated in microcosms at 5 and 17 °C to mimic cloud conditions were analysed by combining LC-MS and NMR; the results were evaluated in comparison to similar suspensions kept at constant temperature. The differences in the metabolome profiles were deciphered using multivariate statistics (PLS-DA).

Results

Key cold shock biomarkers were observed, including cryoprotectants (trehalose, glucose, glycerol, carnitine, glutamate), antioxidants (glutathione and carnitine) and their precursors, alkaloids (bellendine and slaframine) and metabolites involved in energy metabolism (ATP, carbohydrates). Furthermore, new short peptides (nine dipeptides and a tetrapeptide) were found that have no known function.

Conclusions

This study shows that in response to cold temperatures, Pseudomonas syringae PDD-32b-74 demonstrates numerous metabolism modifications to counteract the impacts of low temperatures.

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Acknowledgements

This research has been supported by the ANR French program MetaboHUB. We would like to thank Mélanie Pétéra for her valuable technical assistance.

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Authors and Affiliations

  1. Université Clermont Auvergne – CNRS – SIGMA-Clermont, Institut de Chimie de Clermont-Ferrand, 63000, Clermont-Ferrand, France

    Cyril Jousse, Céline Dalle, Isabelle Canet, Marie Lagrée, Mounir Traïkia, Martine Sancelme, Pierre Amato & Anne-Marie Delort

  2. Université Clermont Auvergne – INRA, UNH, 63000, Clermont-Ferrand, France

    Bernard Lyan

  3. Université Clermont Auvergne – INRA, MetaboHUB/Plateforme d’exploration du métabolisme, Clermont-Ferrand, France

    Cyril Jousse, Céline Dalle, Marie Lagrée, Mounir Traïkia, Bernard Lyan, Cédric Mendes & Anne-Marie Delort

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  1. Cyril Jousse
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  2. Céline Dalle
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Jousse, C., Dalle, C., Canet, I. et al. Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water. Metabolomics 14, 11 (2018). https://doi.org/10.1007/s11306-017-1295-7

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