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Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays

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Abstract

Oxalate in some plants is toxic to vertebrates and causes kidney failure. In the present study, we investigated on the metabolic effectiveness of gamma ray irradiation to seeds of Rumex obtusifolius, which are known to accumulate high level of soluble oxalate, to lower oxalate accumulation in leaves. Reduced rate of germination, discoloration of cotyledons, and deformed true leaves were observed by elevated irradiation. Metabolome analysis of primary metabolites using capillary electrophoresis–mass spectrometry showed a decrease in oxalate contents in the leaves of plants from gamma ray-irradiated seeds. Moreover, organic acids such as malate and 2-oxoglutarate also decreased, whereas amino acids such as glutamate and glutamine increased. These results indicated that seed irradiation by gamma rays leads to dynamic changes in metabolic pathways as well as plant growth/development.

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Acknowledgments

We thank Mr. Y. Kakinuma, Ms. K. Yokoyama and other students (Department of Environmental Science, Faculty of Engineering, Saitama University) for technical help. Edition of this manuscript by Dr. S. M. Uchimiya (USDA-ARS) is appreciated. This research was supported by the Funding Program for Next Generation World-Leading Researchers (NEXT Program).

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Author notes

  1. Issay Narumi

    Present address: Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Oura-gun, Gunma, 374-0193, Japan

Authors and Affiliations

  1. Graduate School of Science and Engineering, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama, Saitama, 338-8570, Japan

    Sayaka Kitano, Atsuko Miyagi & Maki Kawai-Yamada

  2. Medical and Biotechnological Application Division, Quantum Beam Science Directorate, Japan Atomic Energy Agency, 1233 Watanuki, Takasaki, Gunma, 370-1292, Japan

    Yutaka Oono, Yoshihiro Hase & Issay Narumi

  3. Institute for Environmental Science and Technology, Saitama University, 225 Shimo-Okubo, Sakura-ku, Saitama, Saitama, 338-8570, Japan

    Masatoshi Yamaguchi, Hirofumi Uchimiya & Maki Kawai-Yamada

  4. PRESTO, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan

    Masatoshi Yamaguchi

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  1. Sayaka Kitano
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Correspondence to Maki Kawai-Yamada.

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Sayaka Kitano and Atsuko Miyagi equally contributed to this work.

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Kitano, S., Miyagi, A., Oono, Y. et al. Metabolic alterations in leaves of oxalate-rich plant Rumex obtusifolius L. irradiated by gamma rays. Metabolomics 11, 134–142 (2015). https://doi.org/10.1007/s11306-014-0684-4

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  • DOI: https://doi.org/10.1007/s11306-014-0684-4

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