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Targeted metabolomics in an intrusive weed, Rumex obtusifolius L., grown under different environmental conditions reveals alterations of organ related metabolite pathway

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Abstract

This study was intended to analyze the metabolic pathway of Rumex obtusifolius L. (Broad-leaved dock), destructive weeds worldwide, in relation to major environmental factors (light and temperature). It was found that R. obtusifolius can be classified as plants in accumulating major organic acids such as oxalate in leaves and citrate in stems (Miyagi et al., Metabolomics 6:146–155 2010). The organ specific accumulation of certain metabolites was dissected by metabolomics approach in relation to metabolic pathway. Light or dark experiments showed that in the case of the oxalate accumulation, the major or the most dominated pathway was found to be the citrate-isocitrate-oxalate shunt. Furthermore, under the dark and/or low temperature (5°C) leaves showed sustainable growth with normal accumulation of TCA metabolites. Unlike leaves, there was a different pattern of metabolite accumulation in stems. Other metabolites such as amino acids also showed the organ specific alterations under the different ambient environments.

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Acknowledgements

This research was supported by a grant from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN) and the CREST, JST, Japan.

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Authors and Affiliations

  1. Institute for Environmental Science and Technology, Saitama University, 255 Shimo-Okubo, Sakura-Ku, Saitama City, Saitama, 338-8570, Japan

    Atsuko Miyagi, Maki Kawai-Yamada & Hirofumi Uchimiya

  2. Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan

    Atsuko Miyagi, Kentaro Takahara & Hirofumi Uchimiya

  3. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Hideyuki Takahashi & Hirofumi Uchimiya

  4. Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Sakura-Ku, Saitama City, Saitama, 338-8570, Japan

    Maki Kawai-Yamada

  5. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Maki Kawai-Yamada

Authors
  1. Atsuko Miyagi
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  2. Kentaro Takahara
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  3. Hideyuki Takahashi
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  4. Maki Kawai-Yamada
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  5. Hirofumi Uchimiya
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Correspondence to Hirofumi Uchimiya.

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Supplementary data

Relationship between citrate and organic acids or amino acids in stems of R. obtusifolius grown under either light or dark. Data were taken from Fig. 6. W; week(s). *; P < 0.05, **; P < 0.01. (PDF 25 kb)

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Miyagi, A., Takahara, K., Takahashi, H. et al. Targeted metabolomics in an intrusive weed, Rumex obtusifolius L., grown under different environmental conditions reveals alterations of organ related metabolite pathway. Metabolomics 6, 497–510 (2010). https://doi.org/10.1007/s11306-010-0220-0

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