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A high-density linkage map with 2560 markers and its application for the localization of the male-sterile genes ms3 and ms4 in Cryptomeria japonica D. Don

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Abstract

Cryptomeria japonica pollinosis is one of the most serious allergic diseases in Japan; this is a social problem because C. japonica is the most important Japanese forestry species. In order to reduce the amount of pollen dispersed, breeding programs using trees with male-sterile genes have been implemented. High-density linkage maps with stable ordering of markers facilitate the localization of male-sterile genes and the construction of partial linkage maps around them in order to develop markers for use in marker-assisted selection. In this study, a high-density linkage map for C. japonica with 2560 markers was constructed. The observed map length was 1266.2 cM and the mean distance between adjacent markers was 0.49 cM. Using information from this high-density map, we newly located two male-sterile genes (ms3 and ms4) on the first and fourth linkage groups, respectively, and constructed partial linkage maps around these loci. We also constructed new partial linkage maps around the ms1 and ms2 loci using additional SNP markers. The closest markers to the ms1, ms2, ms3, and ms4 male-sterile loci were estSNP04188 (1.8 cM), estSNP00695 (7.0 cM), gSNP05415 (3.1 cM), and estSNP01408 (7.0 cM) respectively. These results allowed us to develop SNP markers tightly linked to the male sterile genes for use in MAS; this will accelerate the future isolation of these genes by map-based cloning approaches.

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Acknowledgments

The authors would like to thank M. Koshiba, Y. Komatsu, Y. Taguchi, and S. Totsuka for the assistance with laboratory work and the sampling of leaves in the nursery. We also thank K. Arai, K. Nemoto, M. Sugiyama, H. Kimura, T. Suzuki, and Y. Hosoya for raising of the plant materials. This work was partly supported by a Grant-in-Aid from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry, Research Grant #201421 of the Forestry and Forest Products Research Institute and JSPS KAKENHI Grant Numbers 25850116 and 25450223.

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Authors and Affiliations

  1. Graduate School of Science and Technology, Niigata University, 8050, Ikarashi 2-Nocho, Nishi-ku, Niigata, 950-2181, Japan

    Yoshinari Moriguchi

  2. Department of Forest Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan

    Kentaro Uchiyama, Saneyoshi Ueno, Tokuko Ujino-Ihara, Asako Matsumoto & Yoshihiko Tsumura

  3. Niigata Prefectural Forest Research Institute, 2249-5 Unotoro, Murakami, Niigata, 958-0264, Japan

    Junji Iwai & Daisuke Miyajima

  4. Toyama Prefectural Agricultural Forestry and Fisheries Research Center, Forestry Research Institute, Yoshimine 3, Tateyama-cho, Nakashinkawagun, Toyama, 930-1362, Japan

    Maki Saito

  5. Faculty of Agriculture, Niigata University, 8050, Igarashi 2-Nocho, Nishi-ku, Niigata, 950-2181, Japan

    Masaya Sato

  6. Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan

    Yoshihiko Tsumura

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  1. Yoshinari Moriguchi
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  2. Kentaro Uchiyama
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  3. Saneyoshi Ueno
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  4. Tokuko Ujino-Ihara
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  5. Asako Matsumoto
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  6. Junji Iwai
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  7. Daisuke Miyajima
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  8. Maki Saito
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  9. Masaya Sato
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  10. Yoshihiko Tsumura
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Correspondence to Yoshihiko Tsumura.

Additional information

Communicated by D. Grattapaglia

Data archiving statement

All marker information and marker/position information from the linkage map were submitted to DDBJ and the TreeGenes Database, respectively.

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Fig. S1

Positions of the 508 common markers mapped on two Cryptomeria japonica linkage maps in Moriguchi et al. (2012) and the present study. Both axes indicate cumulative centimorgans (cM). (JPG 73 kb)

High resolution image (EPS 839 kb)

Fig. S2

Partial linkage maps around the ms2 gene constructed in the present study (right) and that by Moriguchi et al. (2014a) (left). Marker names are indicated to the right of the linkage groups. Centimorgan distances (Kosambi) are indicated to the left of each linkage group. The last letter of the locus indicates the nature of the markers, i.e., S indicates genomic SSRs. (JPG 273 kb)

High resolution image (EPS 1519 kb)

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Moriguchi, Y., Uchiyama, K., Ueno, S. et al. A high-density linkage map with 2560 markers and its application for the localization of the male-sterile genes ms3 and ms4 in Cryptomeria japonica D. Don. Tree Genetics & Genomes 12, 57 (2016). https://doi.org/10.1007/s11295-016-1011-1

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