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Genetic variation and diversity among loquat accessions

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Abstract

Loquat (Eriobotrya japonica (Thunb.) Lindl., Maloideae, Rosaceae) is a subtropical evergreen fruit tree indigenous of China, where the center of origin of the species is located. Loquat is grown in all subtropical areas and was introduced in the Mediterranean basin in late eighteenth century. In Europe, the largest germplasm bank is located at Instituto Valenciano de Investigaciones Agrarias (IVIA; Valencia, Spain). Thirteen microsatellites and a conserved region of S-allele were used to assess the genetic diversity of 102 accessions of the IVIA collection. A total of 38 SSR alleles and 11 putative S-alleles were used to study the genetic structure of the loquat germplasm bank using the STRUCTURE software, Factorial Correspondence Analysis (FCA), and unweighted pair-group method (UPGMA) cluster analyses. The total diversity was H T = 0.5682, the genetic differentiation G ST = 0.1660, and the standardized G ST reached a much higher value of G′ST = 0.4948. The Evanno’s test indicated that the most informative number of populations was five, with accessions distributed according to their geographic origin in two, one, and two groups of Spanish, Italo-Spanish, and non-European origin, respectively. Knowledge of the substructure and diversity of the IVIA loquat collection and the self-incompatibility genotype data will allow us to select and incorporate useful materials into the loquat breeding program.

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Acknowledgments

The authors acknowledge Prof Janick from revision and edition of the manuscript. This research was funded by grant RF 2010-0003-00-00 from the National Agency “Instituto Nacional de Investigaciones Agrarias” (INIA). MBV was funded by a fellowship from INIA.

Data Archiving Statement

The SSR markers used in this study were submitted to the GeneBank; the accession numbers are indicated in Table  2 and reference herein. The results obtained were not susceptible of submission to public databases.

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Authors and Affiliations

  1. Fruit Tree Breeding Department, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113, Moncada, Valencia, Spain

    Manuel Blasco, María del Mar Naval, Elena Zuriaga & María Luisa Badenes

Authors
  1. Manuel Blasco
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  2. María del Mar Naval
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  3. Elena Zuriaga
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  4. María Luisa Badenes
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Corresponding author

Correspondence to María Luisa Badenes.

Additional information

Communicated by D. Chagné

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Table S1

Alleles obtained of the 102 accessions using 13 microsatellite and a conserved region of S-allele (DOC 448 kb)

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Blasco, M., Naval, M.d.M., Zuriaga, E. et al. Genetic variation and diversity among loquat accessions. Tree Genetics & Genomes 10, 1387–1398 (2014). https://doi.org/10.1007/s11295-014-0768-3

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  • DOI: https://doi.org/10.1007/s11295-014-0768-3

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