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Genetic structure and phylogeography of Pyrus pashia L. (Rosaceae) in Yunnan Province, China, revealed by chloroplast DNA analyses

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Abstract

In this study, we investigated two chloroplast intergenic spacers (accD-psaI and trnL-trnF) for a total of 266 individuals over 22 populations of Pyrus pashia to detect the genetic diversity, genetic, and phylogeographic structure and provide needed information for the development of conservation strategies. Thirteen haplotypes (H1–H13) were recognized. A high level of total diversity was detected (H T = 0.746). Analysis of molecular variance indicated that the genetic variation mainly existed within populations, representing 59.61 % of the total variation. Genetic differentiation among populations was high (F st = 0.404). A Mantel test did not show a correlation between the genetic and geographic distances (r = 0.139, P = 0.09, 1,000 permutations), implying that geographic distance was not critical to gene flow among populations. A significantly higher N st than G st (N st = 0.420, G st = 0.402, P < 0.05) reflected the phylogeographic structure in P. pashia. While nested clade analyses of clade 2-2 showed that restricted gene flow existed among populations, clades 1-2 and 2-1, and the total cladogram exhibited contiguous range expansion events in P. pashia. Both sum of square deviations and the raggedness index failed to reject the sudden demographic expansion model. The overall population expansion of P. pashia was estimated to occur between 621,000 and 209,000 years ago.

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Acknowledgments

We sincerely thank En-Sheng Dong form the Fruit and Vegetable Work Station of Weining County, Guizhou Province, China, for his help in sampling P. pashia populations in Guizhou Province. This project was supported by the National Natural Science Foundation of China (no. 30871690).

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Authors and Affiliations

  1. Department of Horticulture, the State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Hangzhou, Zhejiang, 310058, China

    Jing Liu, Ping Sun, Xiaoyan Zheng, Chunyun Hu & Yuanwen Teng

  2. Institute of Horticulture and Landscape, College of Ecology, Lishui University, Lishui, Zhejiang, 323000, China

    Xiaoyan Zheng

  3. Department of Plant Sciences, University of California, Mail Stop 2, Davis, CA, 95616-8780, USA

    Daniel Potter

  4. Institute of Horticulture, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China

    Kunming Li

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  1. Jing Liu
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Correspondence to Yuanwen Teng.

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Communicated by E. Dirlewanger

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Liu, J., Sun, P., Zheng, X. et al. Genetic structure and phylogeography of Pyrus pashia L. (Rosaceae) in Yunnan Province, China, revealed by chloroplast DNA analyses. Tree Genetics & Genomes 9, 433–441 (2013). https://doi.org/10.1007/s11295-012-0564-x

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  • DOI: https://doi.org/10.1007/s11295-012-0564-x

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