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Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea)

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Abstract

The usual red color of young leaves of peach (Prunus persica f. atropurpurea) is due to the accumulation of anthocyanin. Real-time PCR analysis revealed a strong correlation between the expression levels of anthocyanin biosynthetic genes and anthocyanin content in leaves at different developmental stages. The expression profiles of both anthocyanin biosynthetic genes and photorespiratory genes showed significant changes in leaves held in the dark or exposed to heat stress, compared with controls. The expression of anthocyanin biosynthetic genes dramatically decreased in peach red leaves following dark or heat treatments, resulting in a significant decrease of anthocyanin accumulation. However, the photorespiration-related genes GDCH and GOX exhibited increased expression in peach leaves after dark or heat treatment. Moreover, the expression levels of GDCH and GOX in the Arabidopsis chi/f3′h mutant that does not accumulate anthocyanins were higher than in the wild type. Overall, these results support the hypothesis that photorespiration-related genes might be involved in the regulation of anthocyanin biosynthesis. This finding provides a new insight into our understanding of the mechanism underlying the control of anthocyanin biosynthesis in plants.

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Abbreviations

ANS:

Anthocyanidin synthase

bHLH:

Basic-helix-loop-helix

CHI:

Chalcone isomerase

CHS:

Chalcone synthase

DFR:

Dihydroflavonol 4-reductase

F3H:

Flavanone 3-hydroxylase

F3′H:

Flavonoid 3′-hydroxylase

GDCH:

Glycine decarboxylase complex H protein

GOX:

Glycolate oxidase

PAL:

Phenylalanine ammonialyase

PGP:

Phosphoglycolate phosphatase

Q-PCR:

Real-time quantitative PCR

RBCO:

Ribulose-1,5-bisphosphate carboxylase/oxygenase activase

RR:

Prunus persica f. atropurpurea

TFs:

Transcription factors

TPI:

Triosephosphate isomerase

UFGT:

UDP-glucose:flavonoid 3-O-glucosyltransferase

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Acknowledgments

This project was supported by funds received from National Program on Key Basic Research Project of China (973 Program) under grant no. 2011CB100600, the National Natural Science Foundation of China (no. 31101533), and 100 Talents program of the Chinese Academy of Science. Two mutant CHI/F3′H Arabidopsis of CS8586 was kindly offered by The Arabidopsis Information Resource. We would also like to express our gratitude to Juan Li for materials preparation and Dr. Yi Huang for critically reading of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, People’s Republic of China

    Ying Zhou, Jing Li, Jun Cheng, Hui Zhou, Chao Gu & Yue-Peng Han

  2. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People’s Republic of China

    Dong Guo

  3. The New Zealand Institute for Plant & Food Research Limited, Palmerston North, Palmerston North, New Zealand

    Sue Gardiner

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  1. Ying Zhou
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Correspondence to Yue-Peng Han.

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Communicated by A. Abbott

Ying Zhou and Dong Guo contributed to this paper equally.

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Zhou, Y., Guo, D., Li, J. et al. Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea). Tree Genetics & Genomes 9, 265–278 (2013). https://doi.org/10.1007/s11295-012-0552-1

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