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Genetic linkage maps of Populus alba L. and comparative mapping analysis of sex determination across Populus species

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Abstract

White poplar (Populus alba L.) is native to Eurasia and is unexploited for its growth potential and stress-adaptive mechanisms. A better knowledge of its genome will allow for more effective protection and use of critical genetic resources. The main objective of this study was the construction of highly informative P. alba genetic maps. Two genotypes were selected from contrasting natural Italian populations and crossed to generate an F1 mapping pedigree. Amplified fragment length polymorphism and simple sequence repeat markers were used to genotype 141 F1 individuals. The pseudo-testcross strategy was applied for linkage analysis. The generated maps showed good overall colinearity to each other and allowed for a complete alignment with the 19 haploid chromosomes of the Populus genome sequence. The locus that determines sex as a morphological trait was positioned on a non-terminal position of LG XIX of the female parent map. Comparison among Populus species revealed differences in the location of the sex locus on LG XIX as well as inconsistencies in the heterogametic sex. The genetic analysis of the sex locus in P. alba provides insights into sex determination in the genus and is useful for the identification of sex-linked markers and the early assessment of plant gender. Furthermore, these genetic maps will greatly facilitate the study of the genomics of Populus and how it can be exploited in applied breeding programs.

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Acknowledgments

The authors wish to thank Dr. Brian Stanton and Dr. Antoine Harfouche for the critical reading of the manuscript as well as the anonymous referees for their valuable comments. We acknowledge Mrs. Matilde Tamantini for technical assistance in molecular analyses. This work was financially supported by the Commission of the European Communities, contracts N°. QLK5-CT-2000-01377 (ESTABLISH) and QLK5-CT-2002-00953 (POPYOMICS). “It does not necessarily reflect its view and in no way anticipates the Commission’s future policy in this area”.

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Author notes

  1. Giuseppe Scarascia Mugnozza

    Present address: Department of Agronomy, Forestry and Land Use, Agricultural Research Council of Italy, via del Caravita 7/a, 00186, Rome, Italy

  2. Isacco Beritognolo

    Present address: Institute for Mediterranean Agriculture and Forest Systems, National Research Council of Italy, Via Madonna Alta, 128, 06128, Perugia, Italy

Authors and Affiliations

  1. Department of Forest Environment and Resources (DISAFRI), University of Tuscia, Via San Camillo de Lellis snc., 01100, Viterbo, Italy

    Isabella Paolucci, Muriel Gaudet, Isacco Beritognolo, Serena Terzoli, Elena Kuzminsky, Giuseppe Scarascia Mugnozza & Maurizio Sabatti

  2. Unité Amélioration, Génétique et Physiologie Forestières, Centre INRA d’Orléans, 2163 avenue de la Pomme de Pin, CS 40001—Ardon, 45075, Orleans Cedex 2, France

    Véronique Jorge

  3. Department of Crop Production, University of Tuscia, via S. Camillo de Lellis snc., 01100, Viterbo, Italy

    Rosario Muleo

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  1. Isabella Paolucci
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  2. Muriel Gaudet
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Correspondence to Muriel Gaudet or Maurizio Sabatti.

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Communicated by S. González-Martínez

Isabella Paolucci and Muriel Gaudet contributed equally to this research.

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Paolucci, I., Gaudet, M., Jorge, V. et al. Genetic linkage maps of Populus alba L. and comparative mapping analysis of sex determination across Populus species. Tree Genetics & Genomes 6, 863–875 (2010). https://doi.org/10.1007/s11295-010-0297-7

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