Abstract
A large number of simple sequence repeat (SSR) marker-containing genetic maps are available for several Populus species. For aspen however, no SSR-containing map has been published so far. In this study, genetic linkage mapping was carried out with an interspecific mapping pedigree of 61 full-sib hybrids of European × quaking aspen (Populus tremula L. × Populus tremuloides Michx.), using the two-way pseudo-testcross strategy. Amplified fragment-length polymorphism (AFLP) and SSR markers were used for mapping, resulting in the first SSR-containing genetic linkage maps for aspen. The maps allow comparisons with a Populus consensus map and other published genetic maps of the genus Populus. The maps showed good collinearity to each other and to the Populus consensus map and provide a direct link to the Populus trichocarpa genomic sequence. Sex as a morphological trait was assessed in the mapping population and mapped on a non-terminal position of linkage group XIX on the male P. tremuloides map.
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Acknowledgement
We thank D. Krabel (Technical University of Dresden) for helpful and supportive discussions, T. Fenning (Forest Research, Northern Research Station, Roslin, UK) for language editing and A. Tusch (vTI, Großhansdorf, Germany) for her assistance in the DNA laboratories. G. von Wuehlisch is gratefully acknowledged for providing the plant material. The research funding was provided by the DFG (German Research Foundation, ProjectFL263/15-1).
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Pakull, B., Groppe, K., Meyer, M. et al. Genetic linkage mapping in aspen (Populus tremula L. and Populus tremuloides Michx.). Tree Genetics & Genomes 5, 505–515 (2009). https://doi.org/10.1007/s11295-009-0204-2
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DOI: https://doi.org/10.1007/s11295-009-0204-2