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Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis

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Abstract

An early flowering mutant plant of Eucalyptus grandis with normal vegetative growth was found in a nursery in northern Brazil. This mutant plant flowers at approximately 90 days from germination. A cross between a wild-type (normal flowering) tree and the mutant was carried out, generating a progeny of 88 individuals where early flowering segregated in an approximate 1:1 ratio. A genome scan with 100 microsatellite markers distributed across the genome was carried out using bulk segregant analysis (BSA) on two contrasting bulks of 15 plants each. Linkages (LOD>3.0) with a major effect early flowering quantitative trait locus (QTL) were detected and confirmed by a full scale cosegregation analysis for markers EMBRA27, EMBRA60, EMBRA164, EMBRA158, EMBRA91, and EMBRA65. A localized linkage map involving the six loci and the early flowering QTL named Eucalyptus early flowering 1 (Eef1) was constructed belonging to linkage group #2 in the existing microsatellite reference map. The Eef1 locus was mapped between markers EMBRA27 and EMBRA164, with distances of 21.8 and 6.4 cM, respectively. In introgression experiments, these two markers could be successfully used with an expected precision of 98% to select plants carrying the Eef1 mutant allele, assuming no recombination interference in the genomic segment. Early flowering could be a very useful trait both in breeding as well as experimental genetics of Eucalyptus.

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Acknowledgements

We would like to acknowledge Silas Zen and Raimundo Medeiros from Ferro Gusa Carajás S.A. for continued support to this research project. This work was supported by the Brazilian Ministry of Science and Technology, FINEP through the competitive grant GENOLYPTUS, “Brazilian Network of Eucalyptus Genome Research” to D.G. and collaborators and the Brazilian National Research Council, CNPq, with a Ph.D. fellowship to A.A.M. and a research fellowship to D.G.

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  1. A. L. Piacezzi

    Present address: , CAF Arcelor Group Av. Carandaí, 1.115-80 andar Centro, Belo Horizonte (MG), CEP, 30130-915, Brazil

Authors and Affiliations

  1. Plant Genetics Laboratory, Embrapa-Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, 70770-970 DF, Brazil

    A. A. Missiaggia & D. Grattapaglia

  2. Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), University of São Paulo, Piracicaba, SP, Brazil

    A. A. Missiaggia

  3. Ferro Gusa Carajás S.A., Vale do Rio Doce Company, Rua Rafael de Almeida Ribeiro, no 600, Imperatriz, MA, Brazil

    A. L. Piacezzi

  4. Graduate Program in Genomic Sciences and Biotechnology, Universidade Catolica de Brasília, SGAN 916 modulo B, Brasília, 70790-160 DF, Brazil

    D. Grattapaglia

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  1. A. A. Missiaggia
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  3. D. Grattapaglia
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Correspondence to D. Grattapaglia.

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Missiaggia, A.A., Piacezzi, A.L. & Grattapaglia, D. Genetic mapping of Eef1, a major effect QTL for early flowering in Eucalyptus grandis . Tree Genetics & Genomes 1, 79–84 (2005). https://doi.org/10.1007/s11295-005-0011-3

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  • DOI: https://doi.org/10.1007/s11295-005-0011-3

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