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Molecular cloning, identification of GSTs family in sunflower and their regulatory roles in biotic and abiotic stress

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Abstract

Glutathione-S-transferase (GST) genes exist widely in plants and play major role in metabolic detoxification of exogenous chemical substances and oxidative stress. In this study, 14 sunflower GST genes (HaGSTs) were identified based on the sunflower transcriptome database that we had constructed. Full-length cDNA of 14 HaGTSs were isolated from total RNA by reverse transcription PCR (RT-PCR). Sunflower was received biotic stress (Sclerotinia sclerotiorum) and abiotic stress (NaCl, low-temperature, drought and wound). GST activity was measured by using the universal substrate. The results showed that most of the HaGSTs were up-regulated after NaCl and PEG6000-induced stresses, while a few HaGSTs were up-regulated after S. sclerotiorum, hypothermia and wound-induced stressed, and there was correlation between the changes of GST activity and the expression of HaGSTs, indicating that HaGSTs may play regulatory role in the biotic and abiotic stress responses. 14 HaGSTs from sunflower were identified, and the expression of HaGSTs were tissue-specific and played regulatory roles in both stress and abiotic stress.

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Availability of data and materials

The datasets generated and analyzed during the current study are not publicly available due to statutory provisions regarding data- and privacy protection, the dataset supporting the conclusions of this article is available upon reasonable request directed to the corresponding author.

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Acknowledgements

Thanks to Dr. Chen Jishan and Dr. Ma Jun from Heilongjiang Academy of Agricultural Sciences for their help in data analysis.

Funding

This word sponsored by Project of National Specialty Oil Industry Technology System (Grant Number CARS-14-1-20) and Postdoctoral Research Fund of Heilongjiang Academy of Agricultural Sciences (Grant Number LRB 185216).

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Authors and Affiliations

  1. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, 150086, Heilongjiang, China

    Ligong Ma

  2. Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin, 150086, Heilongjiang, China

    Ligong Ma, Yunhua Zhang, Qinglin Meng, Fengmei Shi, Jia Liu & Yichu Li

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  1. Ligong Ma
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  2. Yunhua Zhang
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  3. Qinglin Meng
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  4. Fengmei Shi
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Correspondence to Yunhua Zhang or Qinglin Meng.

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This study was approved by The Ethics Committee of Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences.

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Ma, L., Zhang, Y., Meng, Q. et al. Molecular cloning, identification of GSTs family in sunflower and their regulatory roles in biotic and abiotic stress. World J Microbiol Biotechnol 34, 109 (2018). https://doi.org/10.1007/s11274-018-2481-0

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