Abstract
Shikimic acid (SA) is an industrially important chiral compound used in diverse commercial applications, and the insufficient supply by isolation from plants and expensive chemical synthesis of SA has increased the importance of developing strategies for SA synthesis. In our previous studies, glycerol was observed to be an effective carbon source for SA accumulation in E. coli DHPYAAS-T7, where the PTS operon (ptsHIcrr) and aroL and aroK genes were inactivated, and the tktA, glk, aroE, aroF fbr, and aroB genes were overexpressed. For further investigation of the effects of glycerol aerobic fermentation on SA accumulation in E. coli BL21(DE3), the glpD, glpK genes and tktA, glk, aroE, aroF fbr, aroB genes were overexpressed simultaneously. The results indicated that SA production was increased 5.6-fold, while the yield was increased 5.3-fold over that of parental strain in shake flasks. It is demonstrated that the aerobic fermentation of glycerol associated with glpD and glpK gene overexpression increased glycerol flux, resulting in higher SA accumulation in E. coli BL21(DE3)-P-DK.
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This research was financially supported by the Fundamental Research Funds for the Central Universities (ZL2014SK0035), and the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD).
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Yang Yang and Chao Yuan have contributed equally to this work.
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Yang, Y., Yuan, C., Dou, J. et al. Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol. World J Microbiol Biotechnol 30, 3263–3272 (2014). https://doi.org/10.1007/s11274-014-1753-6
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DOI: https://doi.org/10.1007/s11274-014-1753-6