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Characterization of eubacterial and archaeal community diversity in the pit mud of Chinese Luzhou-flavor liquor by nested PCR–DGGE

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Abstract

The aim of this study was to investigate and compare the microbial community structures of eubacteria and archaea in the pit mud of Chinese Luzhou-flavor liquor from the wall (Cw) and bottom (Cb) of cellar through nested PCR–denaturing gradient gel electrophoresis (DGGE). The Shannon–Wiener index (H) calculated from the DGGE profiles showed that the community diversities of eubacteria and archaea in samples from Cb were almost higher than that from Cw. In addition, cluster analysis of the DGGE profiles revealed that some differences were found in the microbial community structure in samples from different locations. The closely relative microorganisms of all eubacterial 16S rRNA gene sequences fell into four phyla (Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria), including 12 genera and 2 uncultured eubacteria. Moreover, 37.1 % eubacteria were affiliated with Clostridium. Particularly, genus Acinetobacter was absent in all samples from Cb but present in all samples from Cw. The closely relative microorganisms of all archaeal 16S rRNA gene sequences fell into four genera, which included Methanobrevibacter, Methanoculleus, Methanobacterium and Methanosaeta, while the dominant archaea in samples from Cw and Cb were similar. Results presented in this study provide further understanding of the spatial differences in microbial community structure in the pit mud, and is of great importance for the production and quality improvement of Luzhou-flavor liquor.

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Acknowledgments

This work was financially supported by the National Science Foundation of China (31171742).

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Authors and Affiliations

  1. Key Laboratory of Leather Chemistry and Engineering, Education Ministry, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Xiao-Fei Ding, Chong-De Wu, Li-Qiang Zhang, Jia Zheng & Rong-Qing Zhou

  2. National Engineering Research Center of Solid-State Manufacturing, Luzhou, 646000, People’s Republic of China

    Rong-Qing Zhou

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  1. Xiao-Fei Ding
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  2. Chong-De Wu
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  4. Jia Zheng
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Correspondence to Rong-Qing Zhou.

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Ding, XF., Wu, CD., Zhang, LQ. et al. Characterization of eubacterial and archaeal community diversity in the pit mud of Chinese Luzhou-flavor liquor by nested PCR–DGGE. World J Microbiol Biotechnol 30, 605–612 (2014). https://doi.org/10.1007/s11274-013-1472-4

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  • DOI: https://doi.org/10.1007/s11274-013-1472-4

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