Abstract
We studied banana lignocellulosic biomass (BALICEBIOM) that is abandoned after fruit harvesting, and assessed its biochemical methane potential, because of its potential as an energy source. We monitored biogas production from six morphological parts (MPs) of the “Williams Cavendish” banana cultivar using a modified operating procedure (KOP) using KOH. Volatile fatty acid (VFA) production was measured using high performance liquid chromatography. The bulbs, leaf sheaths, petioles–midribs, leaf blades, rachis stems, and floral stalks gave total biogas production of 256, 205, 198, 126, 253, and 221 ml g−1 dry matter, respectively, and total biomethane production of 150, 141, 127, 98, 162, and 144 ml g−1, respectively. The biogas production rates and yields depended on the biochemical composition of the BALICEBIOM and the ability of anaerobic microbes to access fermentable substrates. There were no significant differences between the biogas analysis results produced using KOP and gas chromatography. Acetate was the major VFA in all the MP sample culture media. The bioconversion yields for each MP were below 50 %, showing that these substrates were not fully biodegraded after 188 days. The estimated electricity that could be produced from biogas combustion after fermenting all of the BALICEBIOM produced annually by the Cameroon Development Corporation–Del Monte plantations for 188 days is approximately 10.5 × 106 kW h (which would be worth 0.80–1.58 million euros in the current market). This bioenergy could serve the requirements of about 42,000 people in the region, although CH4 productivity could be improved.
Similar content being viewed by others
Abbreviations
- BMP:
-
Biochemical methane potential
- BALICEBIOM:
-
Banana lignocellulosic biomass
- C/N:
-
Carbon/nitrogen ratio
- CDC:
-
Cameroon Development Corporation
- DM:
-
Dry matter
- DT:
-
Digestion time
- FCFA:
-
Franc des Colonies Françaises d’Afrique
- GE:
-
Glucose equivalent
- GC:
-
Gas chromatography
- HPLC:
-
High performance liquid chromatography
- KOP:
-
Operating procedure using KOH
- MP:
-
Morphological part
- MV:
-
Market value
- SD:
-
Standard deviation
- TOC:
-
Total organic carbon
- TBP:
-
Total biogas production
- TMP:
-
Total methane production
- VFA:
-
Volatile fatty acid
References
AES-SONEL (2013) Société Nationale d’Électricité du Cameroun. http://aessoneltoday.com/decision-arsel-fixant-les-nouveaux-tarifs-delectricite.html. Accessed 10 Jan 2013
Allen SE, Grimshaw HM, Parkinson JA, Quarmby C (1974) Chemical analysis of ecological materials. Blackwell, New York Scientific Publications
Balat M (2011) Production of bioethanol from lignocellulosic materials via the biochemicalpathway. Energ Convers Manage 52:858–875
Barakat A, Monlau F, Steyer JP, Carrere H (2012) Effect of lignin-derived and furan compounds found in lignocellulosic hydrolysates on biomethane production. Bioresour Technol 104:90–99
CEAEQ (2011) Centre d’expertise en analyse environnementale du Québec. Détermination du carbone organique total dans les solides. http://www.ceaeq.gouv.qc.ca/methodes/pdf/MA405C11.pdf. Accessed 26 Jan 2012
Chanakya HN, Sreesha M (2012) Anaerobic retting of banana and arecanut wastes in a plug flow digester for recovery of fiber, biogas and compost. Energ Sust Dev 16:231–235
Chandra R, Takeuchi H, Hasegawa T (2012) Methane production from lignocellulosic agricultural crop wastes. Renew Sust Energ Rev 16:1462–1476
Cheng CL, Lo YC, Lee KS, Lee DJ, Lin CY, Chang JS (2011) Biohydrogen production from lignocellulosic feedstock. Bioresour Technol 102:8514–8523
Chernicharo DLCA (2007) Biological wastewater treatment series. Minas Gerais, Brazil IWA publishing, Anaérobic reactors
CIA (2013) Central Intelligence Agency. https://www.cia.gov/library/publications/the-world-factbook/geos/cm.html. Accessed 10 Jan 2013
Conklin-Brittain NL, Dierenfeld ES, Wrangham RH, Norconk M, Silver SC (1999) Chemical protein analysis: a comparison of Kjeldahl crude protein and total ninhydrin protein from wild, tropical vegetation. J Chem Ecol 24:2601–2622
De Bok FAM, Plugge CM, Stams AJM (2004) Interspecies electron transfer in methanogenic propionate degrading consortia. Water Resour 38:1368–1375
Deublein D, Steinhauser A (2008) Biogas from waste and renewable sources. Wiley-Vch Verlag GmbH and Co, Weinheim
Didderen I, Destain J, Thonart P (2008) Le bioéthanol de seconde génération: la production du bioéthanol à partir de la biomasse lignocellulosique. Les Presses agronomiques de Gembloux, Belgique
FAO (2010) FAOSTAT statistics data base. Agriculture. FAO, Rome
Galbe M, Zacchi G (2002) A review of the production of ethanol from softwood. Appl Microbiol Biotechnol 59:618–628
Gerardi MH (2003) The microbiology of anaerobic digesters. Wiley, New Jersey
Gil MV, Oulego P, Casal MD, Pevida C, Pis JJ, Rubiera F (2010) Mechanical durability and combustion characteristics of pellets from biomass blends. Bioresour Technol 101:8859–8867
Giroux M, Audesse P (2004) Comparaison de deux méthodes de détermination des teneurs en carbone organique, en azote total et du rapport C/N de divers amendements organiques et engrais de ferme. Agrosol Prod Anim 15:107–110
Guo XM, Trably E, Latrille E, Carrère H, Steyer JP (2010) Hydrogen production from agricultural waste by dark fermentation. Int J Hydrogen Energy 35:10660–10673
Hall DO, Rosillo-Calle F (1998) Biomass-other than wood. In: survey of energy resources World energy council, 18th edn. London, pp 227–241
Hamilton C, Hiligsmann S, Beckers L, Masset J, Wilmotte A, Thonart P (2010) Optimization of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode. Int J Hydrogen Energ 35:1089–1098
Hiligsmann S, Masset J, Hamilton C, Beckers L, Thonart P (2011) Comparative study of biological hydrogen production by pure strains and consortia of facultative and strict anaerobic bacteria. Bioresour Technol 102:3810–3818
Kalia VC, Sonakya V, Raizada N (2000) Anaerobic digestion of banana stem waste. Bioresour Technol 73:191–193
Kamdem I, Tomekpe K, Thonart P (2011) Production potentielle de bioéthanol, de biométhane et de pellets à partir des déchets de biomasse lignocellulosique du bananier (Musa spp.) au Cameroun. Biotechnol Agron Soc Environ 15(3): 461–473 http://popups.ulg.ac.be/Base/personne.php?type=auteur&id=7795. Accessed 03 Sept 2011
Lassoudière A (2007) Le bananier et sa culture. Quae, Versailles
Lechien V, Rodriguez C, Ongena M, Hiligsmann S, Rulmont A, Thonart P (2006) Physicochemical and biochemical characterization of non-biodegradable cellulose in Miocene gymnosperm wood from the Entre-Sambre-et-Meuse, Southern Belgium. Org Geochem 37:1465–1476
Liu YQ, Liu Y, Tay JH (2004) The effects of extracellular polymeric substances on the formation and stability of biogranules. Appl Microbiol Biotechnol 65:143–148
Masset J, Hiligsmann S, Hamilton C, Beckers L, Franck F, Thonart P (2010) Effect of pH on glucose and starch fermentation in batchand sequenced-batch mode with a recently isolated strain of hydrogen-producing Clostridium butyricum CWBI1009. Int J Hydrogen Energy 35:3371–3378
Mital KM (1996) Biogas systems: principles and applications. New Age International Publishers Limited, New Delhi
Ogier JC, Ballerini D, Leygue JP, Rigal L, Pourquié J (1999) Production d’éthanol à partir de biomasse lignocellulosique. Oil Gas Sci Technol 54:67–94
Oliveira L, Cordeiro N, Evtuguin DV, Torres IC, Silvestre AJD (2007) Chemical composition of different morphological parts from “Dwarf Cavendish” banana plant and their potential as non-wood renewable source of natural products. Ind Crops Prod 26:163–172
Owen WF, Stuckey DC, JB H Jr, Young LY, McCarty PL (1979) Bioassay for monitoring biochemical methane potential and anaerobic toxicity. Water Resour 13:485–492
Ståhl M, Berghel J (2011) Energy efficient pilot-scale production of wood fuel pellets made from a raw material mix including sawdust and rapeseed cake. Biomass Bioenergy 35:4849–4854
Wang YS, Byrd CS, Barlaz MA (1994) Anaerobic biodegradability of cellulose and hemicellulose in excavated refuse samples using a biochemical methane potential assay. J Ind Microbiol 13:147–153
Ward AJ, Hobbs PJ, Holliman PJ, Jones DL (2008) Optimisation of the anaerobic digestion of agricultural resources. Bioresour Technol 99:7928–7940
Yadvika Santosh Sreekrishnan TR, Kohli S, Rana V (2004) Enhancement of biogas production from solid substrates using different techniques. J Bioresour Technol 95:1–10
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kamdem, I., Hiligsmann, S., Vanderghem, C. et al. Comparative biochemical analysis during the anaerobic digestion of lignocellulosic biomass from six morphological parts of Williams Cavendish banana (Triploid Musa AAA group) plants. World J Microbiol Biotechnol 29, 2259–2270 (2013). https://doi.org/10.1007/s11274-013-1392-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11274-013-1392-3