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Plant growth promoting bacteria in Brachiaria brizantha

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Abstract

Brachiaria brizantha is considered one of the preferred fodders among farmers for having high forage yield and large production of root mass. The association of beneficial bacteria with these grasses can be very valuable in the recovery of the pasture areas with nutritional deficiency. With the aim of studying this possibility, we carried out the sampling of soil and roots of B. brizantha in three areas (Nova Odessa-SP, São Carlos-SP and Campo Verde-MT, Brazil). Seventy-two bacterial strains were isolated and used in tests to evaluate their biotechnological potential. Almost all isolates presented at least one positive feature. Sixty-eight isolates produced analogues of indole-3-acetic acid, ten showed nitrogenase activity when subjected to the method of increasing the concentration of total nitrogen (total N) in the culture medium and sixty-five isolates showed nitrogenase activity when subjected to acetylene reduction technique. The partial sequencing of 16S rRNA of these isolates allowed the identification of seven main groups, with the prevalence of those affiliated to the genus Stenotrophomonas (69 %). At the end, this work elected the strains C4 (Pseudomonadaceae) and C7 (Rhodospirillaceae) as promising organisms for the development of inoculants due to their higher nitrogenase activity.

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Abbreviations

ARA:

Acetylene reduction assay

IAA:

Indole-3-acetic acid

PGPR:

Plant growth-promoting rhizobacteria

LB:

Luria–Bertani

CC:

Combined carbon

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Acknowledgments

Thanks are due to the Sustainable Agriculture Foundation (AGRISUS) for a scholarship to the first author and the financial support to the development of the project: 443-08.

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Authors and Affiliations

  1. Department of Soil Science, Laboratory of Soil Microbiology, São Paulo State University/College of Agriculture, Av. Pádua Dias, 11, CP 09, Piracicaba, SP, 13418-900, Brazil

    Mylenne Calciolari Pinheiro Silva, Aline Fernandes Figueiredo, Fernando Dini Andreote & Elke Jurandy Bran Nogueira Cardoso

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  1. Mylenne Calciolari Pinheiro Silva
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  2. Aline Fernandes Figueiredo
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  3. Fernando Dini Andreote
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  4. Elke Jurandy Bran Nogueira Cardoso
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Correspondence to Mylenne Calciolari Pinheiro Silva or Elke Jurandy Bran Nogueira Cardoso.

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Silva, M.C.P., Figueiredo, A.F., Andreote, F.D. et al. Plant growth promoting bacteria in Brachiaria brizantha . World J Microbiol Biotechnol 29, 163–171 (2013). https://doi.org/10.1007/s11274-012-1169-0

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