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Green coconut fiber: a novel carrier for the immobilization of commercial laccase by covalent attachment for textile dyes decolourization

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Abstract

Commercial laccase formulation was immobilized on modified green coconut fiber silanized with 3-glycidoxypropyltrimethoxysilane, aiming to achieve a cheap and effective biocatalyst. Two different strategies were followed: one point (pH 7.0) and multipoint (pH 10.0) covalent attachment. The influence of immobilization time on enzymatic activity and the final reduction with sodium borohydride were evaluated. The highest activities were achieved after 2 h of contact time in all situations. Commercial laccase immobilized at pH 7.0 was found to have higher activity and higher affinity to the substrate. However, the immobilization by multipoint covalent attachment improved the biocatalyst thermal stability at 50 °C, when compared to soluble enzyme and to the immobilized enzyme at pH 7.0. The Schiff’s bases reduction by sodium borohydride, in spite of causing a decrease in enzyme activity, showed to contribute to the increase of operational stability through bonds stabilization. Finally, these immobilized enzymes showed high efficiency in the continuous decolourization of reactive textile dyes. In the first cycle, the decolourization is mainly due to dyes adsorption on the support. However, when working in successive cycles, the adsorption capacity of the support decreases (saturation) and the enzymatic action increases, indicating the applicability of this biocatalyst for textile wastewater treatment.

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Abbreviations

GPTMS:

3-glycidoxypropyltrimethoxysilane

RB5:

Reactive black 5

RB114:

Reactive blue 114

RY15:

Reactive yellow 15

RY176:

Reactive yellow 176

RR239:

Reactive red 239

RR180:

Reactive red 180

ABTS:

2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt

E :

Enzymatic state 0

E 1 :

Enzymatic state 1

k :

Thermal inactivation parameter

A :

Residual enzyme activity

α:

Ratio of specific activity

t :

Time

t 1/2 :

Half-life time

F :

Stabilization factor

v :

Reaction velocity

v max :

Maximum velocity

K M :

Michaelis–Menten constant

[S]:

ABTS concentration

A i :

Initial absorbance

A f :

Final absorbance

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Acknowledgments

Financial support for this work was in part provided by the project FCT/CAPES/(CAPES 4.1.3/CAPES/CPLP) and by project PEst-C/EQB/LA0020/2011, financed by FEDER through COMPETE—Programa Operacional Factores de Competitividade and by FCT—Fundação para a Ciência e a Tecnologia, Portugal, for which the authors are thankful. The authors also wish to thank Novozymes (Denmark) for laccase from Aspergillus and DyStar (Porto, Portugal) for reactive dyes. S. Silvério thanks FCT for the Ph.D Scholarship (SFRH/BD/43439/2008), M. A. Z. Coelho thanks CNPq and FAPERJ (Brazil) and A. P. M. Tavares acknowledge the financial support (Programme Ciência 2008).

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Authors and Affiliations

  1. Laboratory of Separation and Reaction Engineering (LSRE), Associate Laboratory LSRE/LCM, Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, Rua do Dr. Roberto Frias, 4200-465, Porto, Portugal

    Raquel O. Cristóvão, Sara C. Silvério, Ana P. M. Tavares, José M. Loureiro, Rui A. R. Boaventura & Eugénia A. Macedo

  2. Escola de Química/UFRJ, Av. Horácio Macedo, 2030, CT, Bloco E, Cidade Universitária, Rio de Janeiro, 21941-909, Brazil

    Ana Iraidy S. Brígida & Maria Alice Z. Coelho

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  1. Raquel O. Cristóvão
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  2. Sara C. Silvério
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  3. Ana P. M. Tavares
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  4. Ana Iraidy S. Brígida
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  5. José M. Loureiro
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  6. Rui A. R. Boaventura
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  7. Eugénia A. Macedo
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  8. Maria Alice Z. Coelho
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Corresponding author

Correspondence to Eugénia A. Macedo.

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Cristóvão, R.O., Silvério, S.C., Tavares, A.P.M. et al. Green coconut fiber: a novel carrier for the immobilization of commercial laccase by covalent attachment for textile dyes decolourization. World J Microbiol Biotechnol 28, 2827–2838 (2012). https://doi.org/10.1007/s11274-012-1092-4

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  • DOI: https://doi.org/10.1007/s11274-012-1092-4

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