Abstract
A thermostable laccase was isolated from a tropical white-rot fungus Polyporus sp. which produced as high as 69,738 units of laccase l−1 in an optimized medium containing 20 g of malt extract l−1, 2 g of yeast extract l−1, 1.5 mM CuSO4. The laccase was purified to electrophoretic purity with a final purification of 44.70-fold and a recovery yield of 21.04%. The purified laccase was shown to be a monomeric enzyme with a molecular mass of 60 kDa. The optimum temperature and pH value of the laccase were 75°C and pH 4.0, respectively, for 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS). The Michaelis–Menten constant (K m ) of the laccase was 18 μM for ABTS substrate. The laccase was stable at pH values between 5.5 and 7.5. About 80% of the initial enzyme activity was retained after incubation of the laccase at 70°C for 2 h, indicating that the laccase was intrinsically highly thermostable and with valuable potential applications. The laccase activity was promoted by 4.0 mM of Mg2+, Mn2+, Zn2+ and Ca2+, while inhibited by 4.0 mM of Co2+, Al3+, Cu2+, and Fe2+, showing different profiles of metal ion effects.
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Acknowledgments
We would like to thank the National Natural Science Foundation of China (Grant No. 30671457) and the Project of Science & Technology of Guangdong province (Grant No. 2008B011000005) for financial assistance.
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Guo, LQ., Lin, SX., Zheng, XB. et al. Production, purification and characterization of a thermostable laccase from a tropical white-rot fungus. World J Microbiol Biotechnol 27, 731–735 (2011). https://doi.org/10.1007/s11274-010-0502-8
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DOI: https://doi.org/10.1007/s11274-010-0502-8