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Batch production of Pyranose 2-oxidase from Trametes versicolor (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics

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Abstract

The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and tap water shaken at 150 rpm and 26°C, and the optimum clover straw concentration was determined to be 12 g/l. The effects of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase was used for improving the properties of cotton fabrics.

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References

  • Akkaya A, Pazarlioglu NK (2011) Microbial modification of polyethylene terephthalate fabric. J Appl Polym Sci 121:690–695. doi:10.1002/app.33740

    Article  CAS  Google Scholar 

  • Albrecht M, Lengauer T (2003) Pyranose oxidase identified as a member of the GMC oxidoreductase family. Bioinformatics 19:1216–1220. doi:10.1093/bioinformatics/btg140

    Article  CAS  Google Scholar 

  • Aly AS, Moustafa AB, Hebeish A (2004) Bio-technological treatment of cellulosic textiles. J Clean Prod 12:697–705. doi:10.1016/S0959-6526(03)00074-X

    Article  Google Scholar 

  • Artolozaga MJ, Kubatova E, Volc J, Kalisz HM (1997) Pyranose 2-oxidase from Phanerochaete chrysosporium—further biochemical characterisation. Appl Microbiol Biotechnol 47:508–514

    Article  CAS  Google Scholar 

  • Bannwarth M, Bastian S, Heckmann-Pohl D, Giffhorn F, Schulz GE (2004) Crystal structure of pyranose 2-oxidase from the white-rot fungus Peniophora sp. Biochemistry 43:11683–11690. doi:10.1021/bi048609q

    Article  CAS  Google Scholar 

  • Bergquist LM, Searcy RL (1963) Application of the berthelot reaction for rapid detection of urea hydrolysis by proteus strains. J Bacteriol 85:954–955

    CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Buchert J, Pere J, Poulakk A, Nousiainen P (1998) Enzymatic scouring of cotton. International AATCC conference, Philadelphia

  • Buchert J, Pere J, Puolakka A, Nousiainen P (2000) Scouring of cotton with pectinases, proteases and lipases. Text Chem Color Am D 32:48–52

    CAS  Google Scholar 

  • Costa-Ferreira M, Couto A (2003) High ionic strength tolerance of pyranose oxidase from Trametes versicolor and its purification. Process Biochem 38:1019–1023

    Article  CAS  Google Scholar 

  • Couto SR, Toca-Herrera JL (2006) Laccasses in the textile industry. Biotechnol Mol Biol Rev 1:115–120

    Google Scholar 

  • Csiszar E, Szakacs G, Rusznak I (1998) Combining traditional cotton scouring with cellulose enzymatic treatment. Text Res J 68:163–167

    Article  CAS  Google Scholar 

  • Csiszar E, Losonczi A, Szakacs G, Rusznak I, Bezur L, Reicher J (2001) Enzymes and chelating agent in cotton pretreatment. J Biotechnol 89:271–279

    Article  CAS  Google Scholar 

  • Csiszar E, Losonczi A, Koczka B, Szakacs G, Pomlenyi A (2006) Degradation of lignin-containing materials by xylanase in biopreparation of cotton. Biotechnol Lett 28:749–753. doi:10.1007/s10529-006-9042-6

    Article  CAS  Google Scholar 

  • Daniel G, Volc J, Kubatova E (1994) Pyranose Oxidase, a major source of H2O2 during wood degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida. Appl Environ Microbiol 60:2524–2532

    CAS  Google Scholar 

  • Davulcu A, Anis P, Eren HA (2007) Enzymatic bleaching of cotton. 6th international conference—TEXSCI, June 5–7, Liberec, Czech Republic

  • de Koker TH, Mozuch MD, Cullen D, Gaskell J, Kersten PJ (2004) Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation. Appl Environ Microbiol 70:5794–5800. doi:10.1128/Aem.70.10.5794-5800.2004

    Article  Google Scholar 

  • Elisashvili V, Penninckx M, Kachlishvili E, Tsiklauri N, Metreveli E, Kharziani T, Kvesitadze G (2008) Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition. Bioresour Technol 99:457–462. doi:10.1016/j.biortech.2007.01.011

    Article  CAS  Google Scholar 

  • Erden E, Ucar MC, Kaymaz Y, Pazarlioglu N (2009) New and different lignocellulosic materials from Turkey for laccase and manganese peroxidase production by Trametes versicolor. Eng Life Sci 9:60–65. doi:10.1002/elsc.200700025

    Article  CAS  Google Scholar 

  • Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem 59:257–268

    Article  CAS  Google Scholar 

  • Giffhorn F (2000) Fungal pyranose oxidases: occurrence, properties and biotechnical applications in carbohydrate chemistry. Appl Microbiol Biotechnol 54:727–740

    Article  CAS  Google Scholar 

  • Hartzell MM, Hsieh YL (1998) Enzymatic scouring to improve cotton fabric wettability. Text Res J 68:233–241

    Article  CAS  Google Scholar 

  • Hartzell-Lawson M, Durrant SK (2000) The efficiency of pectinase scouring with agitation to improve cotton fabric wettability. Text Chem Color Am D 32:86–90

    CAS  Google Scholar 

  • Hossain SM, Anantharaman N (2006) Activity enhancement of ligninolytic enzymes of Trametes versicolor with bagasse powder. Afr J Biotechnol 5:189–194

    CAS  Google Scholar 

  • Karmali A, Coelho J (2010) Bioconversion of d-glucose into d-glucosone by Glucose 2-oxidase from Coriolus versicolor at moderate pressures. Appl Biochem Biotechnol. doi:10.1007/s12010-010-9094-x

  • Leitner C, Volc J, Haltrich D (2001) Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor. Appl Environ Microbiol 67:3636–3644

    Article  CAS  Google Scholar 

  • Lin CH, Hsieh YL (2001) Direct scouring of greige cotton fabrics with proteases. Text Res J 71:425–434

    Article  CAS  Google Scholar 

  • Lorenzo M, Moldes D, Couto SR, Sanroman A (2002) Improving laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor. Bioresour Technol 82:109–113

    Article  CAS  Google Scholar 

  • Moredo N, Lorenzo M, Dominguez A, Moldes D, Cameselle C, Sanroman A (2003) Enhanced ligninolytic enzyme production and degrading capability of Phanerochaete chrysosporium and Trametes versicolor. World J Microbiol Biotechnol 19:665–669

    Article  CAS  Google Scholar 

  • Papinutti VL, Diorio LA, Forchiassin F (2003) Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran. J Ind Microbiol Biotechnol 30:157–160. doi:10.1007/s10295-003-0025-5

    Article  CAS  Google Scholar 

  • Pazarlioglu NK, Akkaya A, Tahsinsoy D (2009) Pyranose 2-oxidase (P2O): production from Trametes versicolor in stirred tank reactor and its partial characterization. Prep Biochem Biotechnol 39:32–45. doi:10.1080/10826060802589569

    Article  CAS  Google Scholar 

  • Pisanelli I, Kujawa M, Spadiut O, Kittl R, Halada P, Volc J, Mozuch MD, Kersten P, Haltrich D, Peterbauer C (2009) Pyranose 2-oxidase from Phanerochaete chrysosporium-Expression in E. coli and biochemical characterization. J Biotechnol 142:97–106. doi:10.1016/j.jbiotec.2009.03.019

    Article  CAS  Google Scholar 

  • Rowell RM, Young RA, Rowell J (1996) Paper and composites from agro-based resources. CRC Press, Boca Raton

    Google Scholar 

  • Sahin UK, Gursoy NC (2003) Enzymatic scouring of cotton fabric. Textile science 5th ınternational conference, Liberec Technical University, Liberec, Czech Repub

  • Tappi Methods (1983) Acid insoluble lignin in wood and pulp. T222 om-83

  • Tzanov T, Costa SA, Gubitz GM, Cavaco-Paulo A (2002) Hydrogen peroxide generation with immobilized glucose oxidase for textile bleaching. J Biotechnol 93:87–94

    Article  CAS  Google Scholar 

  • Tzanov T, Basto C, Gubitz GM, Cavaco-Paulo A (2003) Laccases to improve the whiteness in a conventional bleaching of cotton. Macromol Mater Eng 288:807–810. doi:10.1002/mame.200300100

    Article  CAS  Google Scholar 

  • Valle LJ, Onos M, Garriga P, Calafell M, Schnitzhofer W, Guebitz GM (2006) Bioscouring of cotton fiber with polygalactuonase induced in sclerotium rolfsii using cellulose and glucose-pectin. Text Res J 76:400–405

    Article  Google Scholar 

  • Verenich S, Arumugam K, Shim E, Pourdeyhimi B (2007) Effect of cellulase pretreatment of raw and bleached cotton fibers on properties of hydroentangled nonwoven fabrics. J Appl Polym Sci 105:492–499. doi:10.1002/App.26158

    Article  CAS  Google Scholar 

  • Yachmenev VG, Bertoniere NR, Blanchard EJ (2001) Effect of sonication on cotton preparation with alkaline pectinase. Text Res J 71:527–533

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This project was supported by TUBITAK (Turkish Scientific Technical Research Consultation) (Project No: 104M420).

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Authors and Affiliations

  1. Faculty of Science, Biochemistry Department, Ege University, 35100, Bornova, Izmir, Turkey

    Nurdan Kasikara Pazarlioglu, Emre Erden, M. Cigdem Ucar & Alper Akkaya

  2. Faculty of Engineering, Textile Engineering Department, Dokuz Eylul University, Buca, Izmir, Turkey

    A. Merih Sariisik

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  1. Nurdan Kasikara Pazarlioglu
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  2. Emre Erden
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  3. M. Cigdem Ucar
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  4. Alper Akkaya
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  5. A. Merih Sariisik
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Correspondence to Nurdan Kasikara Pazarlioglu.

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Pazarlioglu, N.K., Erden, E., Ucar, M.C. et al. Batch production of Pyranose 2-oxidase from Trametes versicolor (ATCC 11235) in medium with a lignocellulosic substrate and enzymatic bleaching of cotton fabrics. World J Microbiol Biotechnol 28, 1523–1531 (2012). https://doi.org/10.1007/s11274-011-0955-4

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  • DOI: https://doi.org/10.1007/s11274-011-0955-4

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