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Isolation and characterization of marine bacteria capable of utilizing phthalate

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Abstract

Eleven phthalate-degrading bacterial strains were isolated from seawater collected off the coast of Japan. The isolates were found to be most closely related to the marine bacterial genera Alteromonas, Citreicella, Marinomonas, Marinovum, Pelagibaca, Rhodovulum, Sulfitobacter, Thalassobius, Thalassococcus, Thalassospira, and Tropicibacter. For the first time, members of these genera were shown to be capable of growth on phthalate. The plate assay for visual detection of phthalate dioxygenase activity and PCR detection of a possible gene encoding 4,5-dihydroxyphthalate decarboxylase indicated that phthalate is degraded via 4,5-dihydroxyphthalate to protocatechuate in all the isolates.

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Acknowledgments

We thank for P. Lau for carefully reading the manuscript. This work was financially supported in part by JSPS.KAKENHI (22780077) and by the Kansai University Research Grants: Grant-in-Aid for Encouragement of Scientists 2011.

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Authors and Affiliations

  1. Department of Life Science and Biotechnology and ORDIST, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka, 564-8680, Japan

    Hiroaki Iwaki, Ayaka Nishimura & Yoshie Hasegawa

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  1. Hiroaki Iwaki
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  2. Ayaka Nishimura
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  3. Yoshie Hasegawa
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Correspondence to Hiroaki Iwaki.

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Iwaki, H., Nishimura, A. & Hasegawa, Y. Isolation and characterization of marine bacteria capable of utilizing phthalate. World J Microbiol Biotechnol 28, 1321–1325 (2012). https://doi.org/10.1007/s11274-011-0925-x

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  • DOI: https://doi.org/10.1007/s11274-011-0925-x

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