Abstract
The effect of three levels of oxygen (normal atmosphere (21% O2), low oxygen (16% O2) and enriched oxygen (26% O2)) on the production and germination of conidia by Beauveria bassiana was evaluated using rice as a substrate. The maximum yield of conidia was achieved under hypoxia (16% O2) after 8 days of culture (1.51 × 109 conidia per gram of initial dry substrate), representing an increase of 32% compared to the normal atmosphere. However, germination was reduced by at least 27% due to atmospheric modifications. Comparison of antioxidant enzyme activity (superoxide dismutases and catalases) with the oxidation profiles of biomolecules (proteins and lipids) showed that a decrease in catalase activity in the final days of culture coincided with an increase in the amount of oxidized lipids, showing that oxidative stress was a consequence of pulses of different concentrations of O2. This is the first study describing oxidative stress induction by atmospheric modification, with practical implications for conidia production.
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Acknowledgments
Garza-López, P. M. (Reg. No. 203464) thanks the Mexican National Council for Science and Technology (CONACyT) for the scholarship. This study was financed by the Universidad Autónoma Metropolitana-Iztapalapa and Red PROMEP-Mexico. The authors wish to thank Dr. Wilhelm Hansberg and Dr. Pablo Rangel for their technical assistance in the measurement of catalase activity.
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Garza-López, P.M., Konigsberg, M., Gómez-Quiroz, L.E. et al. Physiological and antioxidant response by Beauveria bassiana Bals (Vuill.) to different oxygen concentrations. World J Microbiol Biotechnol 28, 353–359 (2012). https://doi.org/10.1007/s11274-011-0827-y
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DOI: https://doi.org/10.1007/s11274-011-0827-y