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Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholoma lobynsis

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Abstract

The macrofungus, Tricholoma lobynsis, was chosen to remedy Zn–Cd–Pb contaminated soil. To enhance its metal-extracting efficiency, two heavy metal resistant microbes M6 and K1 were applied owing to their excellent abilities to solubilize heavy metal salts. The two isolated microbial strains could also produce indole acetic acid (IAA), siderophore and solubilize inorganic phosphate, but neither of them showed 1-aminocyclopropane-1-carboxylate deaminase activity. The strains M6 and K1 were identified as Serratia marcescens and Rhodotorula mucilaginosa based on 16S rDNA and ITS sequence analysis respectively. Pot experiment showed that spraying to T. lobynsis-inoculated soil with M6 and K1 respectively could increase total Cd accumulations of this mushroom by 216 and 61%, and Zn by 153 and 49% compared to the uninoculated control. Pb accumulation however, was too low (<1 mg kg−1) to be determined. The results illustrated that special microbes and macrofungi can work together to remedy polluted soil as plant and plant growth promoting microbes do, probably because of excellent metal-accumulating abilities of macrofungi and IAA-siderophore production, phosphate solubilization abilities of the assisted-microbes. This kind of macrofungi-microbe interaction can be developed into a novel bioremediation strategy.

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Acknowledgments

We acknowledge financial support from the National High Technology Research and Development Program of China (863 Program), Project No. 2006AA06Z361 and appreciate special aid from Longquanyi Science and Technology Burea, Chengdu.

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Authors and Affiliations

  1. Key Laboratory for Bio-resources and Eco-environment of Education Ministry, College of Life Science, Sichuan University, Chengdu, 610064, China

    Ling-yun Ji, Wei-wei Zhang, Dong Yu, Yan-ru Cao & Heng Xu

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  1. Ling-yun Ji
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  2. Wei-wei Zhang
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  3. Dong Yu
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  4. Yan-ru Cao
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  5. Heng Xu
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Correspondence to Heng Xu.

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Ji, Ly., Zhang, Ww., Yu, D. et al. Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholoma lobynsis . World J Microbiol Biotechnol 28, 293–301 (2012). https://doi.org/10.1007/s11274-011-0819-y

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  • DOI: https://doi.org/10.1007/s11274-011-0819-y

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