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Susceptibility of Sclerotinia sclerotiorum strains different in oxalate production to infection by the mycoparasite Coniothyrium minitans

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Abstract

Three strains of Sclerotinia sclerotiorum, namely Ep-1PB (PB), Ep-1PK (PK) and Ep-1PNA5 (A5), were compared for the production of oxalic acid (OA) on potato dextrose agar (PDA) and Maxwell agar medium (MAM) and for mycelial susceptibility to infection by the mycoparasite Coniothyrium minitans on PDA. Results showed that strain PB produced negligible oxalate, whereas strain PK was detected to produce oxalate, but much less than that produced by strain A5. The three investigated strains differed slightly in mycelial growth rates and mycelial biomass on PDA. However, colonies of strains PB and PK formed on PDA were more susceptible to invasion by C. minitans than colonies of strain A5. Meanwhile, amendment of synthetic oxalate in PDA at 0.25–2.00 mg g−1 medium suppressed aggressiveness of C. minitans in invasion of colonies of S. sclerotiorum strain PB developed on this medium. These results suggest that infection of hyphae of S. sclerotiorum is negatively affected by the presence of oxalate. The importance of oxalate degradation by C. minitans in its mycoparasitism on hyphae of S. sclerotiorum provides a clue for improvement of the biocontrol efficacy of C. minitans in the future.

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Acknowledgments

This research was funded by a grant from the Natural Science Foundation of China (Grants Nos. 30971953 and 31000877).

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Authors and Affiliations

  1. The State Key Laboratory of Agricultural Microbiology and the Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China

    Yongbing Huang, Xiaoli Xie, Long Yang, Jing Zhang, Guoqing Li & Daohong Jiang

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  1. Yongbing Huang
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  2. Xiaoli Xie
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  3. Long Yang
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  4. Jing Zhang
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  5. Guoqing Li
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Correspondence to Guoqing Li.

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Fig. 1S

Difference in acidification of potato dextrose agar (PDA) in cultures of strains Ep-1PB, Ep-1PK and Ep-1PNA5 of Sclerotinia sclerotiorum (20°C, 48 h). PDA was amended with bromophenol blue (0.1%, w/v). Yellow/brown color indicates decrease in the ambient pH value caused by production of oxalic acid by S. sclerotiorum. Areas of high, moderate and no/slight acidification in cultures were labeled with white, gray and black bars, respectively. (TIFF 4082 kb)

Fig. 2S

A schematic diagram showing the method for sampling agar plugs containing mycelia of Sclerotinia sclerotiorum (Ss) and/or Coniothyrium minitans (Cm) in dual cultures of the two fungi. The sampled agar plugs were individually transferred to potato dextrose agar and incubated at 20°C for 10 days. The colony (S. sclerotiorum alone or C. minitans alone) or the complex colony (S. sclerotiorum + C. minitans) developed from each mycelial agar plug was identified. (TIFF 2898 kb)

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Huang, Y., Xie, X., Yang, L. et al. Susceptibility of Sclerotinia sclerotiorum strains different in oxalate production to infection by the mycoparasite Coniothyrium minitans . World J Microbiol Biotechnol 27, 2799–2805 (2011). https://doi.org/10.1007/s11274-011-0757-8

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