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Effect of carbon, nitrogen sources and inducers on ligninolytic enzyme production by Morchella crassipes

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Abstract

The effect of different carbon, nitrogen sources and inducers on growth and ligninolytic activity by Morel mushroom Morchella crassipes was investigated. The maximum growth was observed in mineral salts broth containing glucose as the carbon source and sodium nitrate as the nitrogen source. Among the inducers, chemical inducers inhibited the growth whereas in natural substrates, growth was not affected much. Manganese peroxidase and lignin peroxidase activity were not detected in the medium with different carbon and nitrogen sources, whereas laccase activity varied depending on carbon source (0.7–3.48 U/ml). Among the inducers, natural inducers resulted in an increase in the enzyme activities. Maximum laccase activity was observed in rice straw (12. 6 U/ml) followed by ABTS (11.6 U/ml); Manganese peroxidase activity was maximum in rice straw (14.32 U/l) wheat straw (12.16 U/l) and phenol red (15 U/l) as the inducers, whereas for Lignin peroxidase activity, rice straw (22 U/l), wheat straw (16 U/l) and veratrylalcohol (20 U/l) served as the best inducers.

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References

  • Ardon O, Kerem Z, Hadar Y (1998) Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton stalk extract. Can J Microbiol 44:676–680

    Article  CAS  Google Scholar 

  • Arora DS, Gill PK (2000) Laccase production by some white rot fungi under different nutritional conditions. Bioresour Technol 73:283–285

    Article  CAS  Google Scholar 

  • Arora DS, Gill PK (2005) Production of ligninolytic enzymes by Phlebia floridensis. World J Microbiol Biotechnol 21:1021–1028

    Article  CAS  Google Scholar 

  • De Groot PWJ, Visser J, Van Griensven LJLD, Schaap PJ (1998) Biochemical and molecular aspects of growth and fruiting of the edible mushroom Agaricus bisporus. Mycol Res 102:1297–1308

    Article  Google Scholar 

  • Eggert C, Temp U, Eriksson KE (1996) The liginolytic system of the white rot fungus Pycnoporous cinnabarinus: purification and characterization of the laccase. Appl Environ Microbiol 602:1151–1158

    Google Scholar 

  • Elisashvili V, Parlar H, Kachlishvili E, Chichua D, Kvesitadze G (2001) Lignocellulolytic activity of Basidiomycetes grown under submerged and solid-state fermentation on plant raw material. Adv Food Sci 23:117–123

    CAS  Google Scholar 

  • Elisashvili V, Kachlishvili E, Tsiklauri N, Bakradze M (2002) Physiological regulation of edible and medicinal higher Basidiomycetes lignocellulolytic enzymes activity. Int J Med Mushroom 4:159–166

    CAS  Google Scholar 

  • Galhaup C, Wagner H, Hinterstoisser B, Haltrich D (2002) Increased production of laccase by the wood degrading basidiomycete Trametes pubescens. Enzyme Microb Technol 30:529–536

    Article  CAS  Google Scholar 

  • Guler P, Arkan O (1999) Cultural characteristics of Morchella esculenta mycelium on some nutrients. Turk J Biol 24:783–794

    Google Scholar 

  • Koroleva OV, Gavrilova VP, Stepanocva EV, Lebedeva VI, Sverdlova NI, Landesman EO et al (2002) Production of lignin modifying enzymes by co-cultivated white rot fungi Cerrena maxima and Coriolus hirsutus and characterization of laccase from Cerrena maxima. Enzyme Microb Technol 30:573–580

    Article  CAS  Google Scholar 

  • Langfelder K, Jahn B, Gehringer H, Schmidt A, Wanner G, Brakhage AA (1998) Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Med Microbiol Immunol 187:79–89

    Article  CAS  Google Scholar 

  • Leonowicz A, Cho NS, Luterek J, Wilkolazka A, Wotjas-Wasilewska M, Matuszewska A, Hofrichter M, Wesenberg D, Rogalski J (2001) Fungal laccase: properties and activity on lignin. J Basic Microbiol 41:185–227

    Article  CAS  Google Scholar 

  • Mikiashvili N, Wasser SP, Nevo E, Elisashvili V (2006) Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity. World J Microbiol Biotechnol 22:999–1002

    Article  CAS  Google Scholar 

  • Niladevi KN, Prema P (2008) Effect of inducers and process parameters on laccase production by Streptomyces psammoticus and its application in dye decolourisation. Biores Technol 99:4583–4589

    Article  CAS  Google Scholar 

  • Nitha B, Meera CR, Janardhanan KK (2007) Anti-inflammatory and antitumour activities of cultured mycelium of morel mushroom, Morchella esculenta. Curr Sci 92(2):235–239

    Google Scholar 

  • Papinutti L, Lechner B (2008) Influence of the carbon source on the growth and lignocellulolytic enzyme production by Morchella esculenta strains. J Ind Microbiol Biotechnol 35:1715–1721

    Article  CAS  Google Scholar 

  • Prasad P, Chauhan K, Kandari LS, Maikhuri RK, Purohit A, Bhatt RP, Rao KS (2002) Morchella esculenta (Guchhi): need for scientific intervention for its cultivation in Central Himalaya. Curr Sci 82:1098–1100

    Google Scholar 

  • Spiker JK, Crawford DL, Thiel EC (1992) Oxidation of phenolic and non-phenolic substrate by the lignin peroxidase of Streptomyces viridosporus T7A. Appl Microbiol Biotechnol 37:518–523

    Article  CAS  Google Scholar 

  • Tien M, Kirk TK (1984) Lignin degrading enzyme from Phanerochaete chrysosporium: purification, characterisation and catalytic properties of a unique-H2O2 requiring oxygenase. Proc Natl Acad Sci USA 81:2280–2284

    Article  CAS  Google Scholar 

  • Ulmer DC, Leisola MSA, Fiechter A (1984) Possible induction of the lignolytic system of Phanerochaete chrysosporium. J Biotech 1:13–24

    Article  CAS  Google Scholar 

  • Vaiterbo A, Yagen B, Mayer AM (1993) Cucurbitacins, attack enzymes and laccase in Botrytis cinerea. Phytochemistry 32:61–65

    Article  Google Scholar 

  • Volk TJ, Leonard TJ (1990) Cytology of the life-cycle of Morchella. Mycol Res 94(3):399–406

    Article  Google Scholar 

  • Wariishi H, Valli K, Gold MH (1992) Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. J Biol Chem 267:23688–23695

    CAS  Google Scholar 

  • Winder RS (2006) Cultural studies of Morchella elata. Mycol Res 110:612–623

    Article  Google Scholar 

  • Zhang GP, Zhang F, Ru WM, Han JR (2009) Solid state fermentation of cornmeal with the ascomycete Morchella esculenta for degrading starch and upgrading nutritional value. World J Microbiol Biotechnol 26:15–20

    Article  Google Scholar 

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Correspondence to M. Sudhakara Reddy.

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Kanwal, H.K., Reddy, M.S. Effect of carbon, nitrogen sources and inducers on ligninolytic enzyme production by Morchella crassipes . World J Microbiol Biotechnol 27, 687–691 (2011). https://doi.org/10.1007/s11274-010-0507-3

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