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Decolorization of an industrial effluent by free and immobilized cells of Stenotrophomonas maltophilia AAP56. Implementation of efficient down flow column reactor

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Abstract

A bacterial strain AAP56, isolated from a polluted soil (from Kelibia city) and identified as Stentrophomonas maltophilia, was particularly interesting for its ability to decolorize recalcitrant dyes of an industrial effluent: SITEX Black. The final percentage decolorization 60% was shown by bacterial culture after incubation in LB medium at 30°C under shaking conditions. The decolorization was closely correlated with the metabolic bacterial growth. The replacement of yeast extract in LB medium composition by soya flour was clearly efficient to enhance the percentage decolorization by 20% and also to reduce the growth medium cost 60-fold. The bacteria were able to reduce 23% from the initial COD and 28% from the initial BOD5 of the effluent. The immobilization of bacterial cells in calcium alginate beads improved by 25% the effectiveness of the biotransformation within 24 h in batch conditions. The potential of a downflow fixed column reactor (DFCR) to decolorize SITEX Black was evaluated under dilution rate. The best decolorization percentage (82%) was recorded at 0.3 h−1. This bioprocess seems to be a potentially useful method to remediate the colored textile wastewater.

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Acknowledgments

The authors gratefully thank Mr Foued El Aïni (Centre International de Technologies de l’Environnement de Tunisie) for COD and BOD analysis and SITEX (Société Industrille de TEXtile, Sousse, Tunisia) Industry to generous gives us the SN effluent. This work was supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology (Financial project of Bioengineering Unit 99 UR/09-26).

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Authors and Affiliations

  1. Biological Engineering Unit 99UR09-26, National Institute of Applied Science and Technology, North urban center B.P.676, 1080, Tunis Cedex, Tunisia

    Said Galai & M. Nejib Marzouki

  2. Interaction Leguminous Micro-organisms Laboratory, Biotechnology Centre of Bordj Cedria B.P.95, 2050, Hammam Lif, Tunisia

    Ferid Limam

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  1. Said Galai
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  2. Ferid Limam
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  3. M. Nejib Marzouki
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Correspondence to Said Galai.

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Galai, S., Limam, F. & Marzouki, M.N. Decolorization of an industrial effluent by free and immobilized cells of Stenotrophomonas maltophilia AAP56. Implementation of efficient down flow column reactor. World J Microbiol Biotechnol 26, 1341–1347 (2010). https://doi.org/10.1007/s11274-010-0306-x

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  • DOI: https://doi.org/10.1007/s11274-010-0306-x

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