Abstract
Three bacterial strains have been isolated from soil in which soybean had been continuously cropped and treated with Opera®, a fungicide containing epoxyconazole and pyraclostrobin. The three strains (1,805, 2,801 and 3,803), obtained from soil at 80–100 cm depth, were selected on medium containing 0.03% Opera®. Morphological examination revealed that the strains were Gram-negative, and two of them (1,805 and 2,801) exhibited polymorphism. The growth profiles demonstrated that 1,805 and 3,803 were more efficient growing in the presence of Opera® than 2,801. Maximum growth was reached between 24 and 48 h, however, 2,801 was not able to survive after this period. The total protein content produced by 1,805, 2,801 and 3,803 in liquid selective medium containing Opera® were 111.0 ± 0.02, 80.0 ± 0.05 and 130.5 ± 0.07 μg/ml, respectively. According to its biochemical and molecular features, strain 1,805 was identified as Klebsiella sp. On the basis of the characteristics presented (facultative anaerobic nature, polymorphic character and capacity of growing in the presence of Opera®) strain 1,805 seems to be able to degrade the epoxyconazole and pyraclostrobin.
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References
Arias-Estévez M, López-Periago E, Martínez-Carballo E, Simal-Gándara J, Mejuto JC, García-Río L (2008) The mobility and degradation of pesticides in soils and the pollution of groundwater resources. Agric Ecosyst Environ 123:247–260
Castillo MA, Felis N, Aragon P, Cuesta G, Sabater C (2006) Biodegradation of the herbicide diuron by Streptomyces isolated from soil. Int Biodeter Biodegr 58:196–202
Cocco P (2002) On the rumors about the silent spring: review of the scientific evidence linking occupational and environmental pesticide exposure to endocrine disruption health effects. Caderno de Saúde Pública 18:379–402
Döbereiner J, Baldani VLD, Baldani JI (1995) Como isolar e identificar bactérias diazotróficas de plantas não-leguminosas. EMBRAPA-SPI, Brasília 60 p
Drancourt M, Bollet C, Carta A, Rousselier P (2001) Phylogenetic analyses of Klebsiella species delineate Klebsiella and Raoultella gen. nov., with description of Raoultella ornithinolytica comb. nov., Raoultella terrigena comb. nov. and Raoultella planticola comb. nov. Int J Syst Evol Microbiol 51:925–932
Environmental Protection Agency—EPA (2006) Office of prevention, pesticides and toxic substances: pesticide fact sheet. EPA, Washington, DC
European Commission (1997). Substances which may not be included as active ingredients in approved pesticide products, Chap. 15, Sect. 2, subsection one, Appendix 5
Food and Agriculture Organization of the United Nations (2008) Feeding the world: sustainable management of natural resources. Environmental Assessment and Management Unit (NRCE), Rome
Gianfreda L, Rao MA (2004) Potential of extra cellular enzymes in remediation of polluted soil: a review. Enz Microb Technol 35:339–354
Grimont PAD, Grimont F (2005) Genus XVI. Klebsiella. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology, 2nd edn, vol 2 (The Proteobacteria), part B (The Gammaproteobacteria). Springer, New York, pp 685–693
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
Harada N, Takagi K, Harazono A, Fujii K, Iwasaki A (2006) Isolation and characterization of microorganisms capable of hydrolyzing the herbicide mefenacet. Soil Biol Biochem 38:173–179
Jones JG, Edington MA (1968) An ecologic survey of hydrocarbon-oxidizing microorganisms. J Genet Microbiol 52:381–390
Karadimos DA, Karaoglanidis GS, Tzavella-Klonari K (2005) Biological activity and physical modes of action of the Qo inhibitor fungicides trifloxystrobin and pyraclostrobin against Cercospora beticola. Crop Protection 24:23–29
Peres F, Moreira JC (2007) Saúde e ambiente em sua relação com o consumo de agrotóxicos em um pólo agrícola do Estado do Rio de Janeiro, Brasil. Cad Saúde Pública 23:5612–5621
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, New York
Singh R, Paul D, Jain RK (2006) Biofilms: implications in bioremediation. Trends Microbiol 14:389–397
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
Ueta J, Pereira NL, Shuhama IK, Cerdeira AL (1999) Biodegradação de Herbicidas e Biorremediação. Biotecnologia Ciência & Desenvolvimento 10:10–13
Ueta J, Shuhama IK, Cerdeira AL (2001) Biodegradação de Herbicidas e Biorremediação: Microrganismos degradadores de atrazina provenientes de solos da região do Aquífero Guarani. Revista Plantio Direto 24:25–30
Veiga MM, Silva DM, Veiga LB, Faria MV (2006) Pesticide pollution in water systems in a small rural community in Southeast Brazil. Cad Saude Publica 22:2391–2399 (in Portuguese)
Wilson K (1990) Preparation of genomic DNA from bacteria–miniprep of bacterial genomic DNA. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology, vol 1. Wiley, New York, pp 241–242
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Flavio Marques Lopes thanks CAPES for fellowship support.
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Lopes, F.M., Batista, K.A., Batista, G.L.A. et al. Biodegradation of epoxyconazole and piraclostrobin fungicides by Klebsiella sp. from soil. World J Microbiol Biotechnol 26, 1155–1161 (2010). https://doi.org/10.1007/s11274-009-0283-0
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DOI: https://doi.org/10.1007/s11274-009-0283-0