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Characterization of 1-aminocyclopropane-1-carboxylate deaminase producing methylobacteria from phyllosphere of rice and their role in ethylene regulation

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Abstract

The presence of 1-aminocyclopropane-1-carboxylate deaminase (ACCD) activity among the phyllosphere methylobacteria of rice was detected and its role in regulating plant ethylene level was assessed. Eighteen methylobacterial isolates from four different cultivars of rice were isolated and screened for ACCD. The 16S rRNA homology of ACCD positive methylobacterial isolate closely related to the species Methylobacterium radiotolerans. The accD gene sequence homology of the isolate was 98% similar to Rhizobium leguminosarum. Foliar spray of ACCD positive methylobacterial isolates enhanced the root and shoot length of rice and tomato seedlings under gnotobiotic condition and lower the ethylene level (60–80%) in the plant species.

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  1. Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India

    C. Chinnadurai, D. Balachandar & S. P. Sundaram

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  1. C. Chinnadurai
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  2. D. Balachandar
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  3. S. P. Sundaram
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Chinnadurai, C., Balachandar, D. & Sundaram, S.P. Characterization of 1-aminocyclopropane-1-carboxylate deaminase producing methylobacteria from phyllosphere of rice and their role in ethylene regulation. World J Microbiol Biotechnol 25, 1403–1411 (2009). https://doi.org/10.1007/s11274-009-0027-1

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