Skip to main content
SpringerLink
Log in

Induction of cellulase gene transcription by a novel oligosaccharide: molasses alcohol stillage substance

  • Short Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

A novel cellulase inducer named MASS was isolated from molasses alcohol stillage (MAS). HPLC analysis identified it as a novel hetero-oligosaccharide composed of the monosaccharides glucose, fructose and xylose in the ratio of 6:3:1; size-exclusion chromatography determined its molecular weight about 4,200 Da. MASS induced both the gene transcription and enzyme activities of cellobiohydrolase (EC 3.2.1.91) (CBH) in the cellulolytic fungus Trichoderma reesei. Cellulase activity assays showed that MASS induced a much stronger CBH activity than cellobiose and lactose, but a weaker one than sophorose. Real-time PCR revealed that MASS and other inducers regulate cbh gene transcription and the induction of gene transcription was correlated with the change of protein activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Beguin P (1990) Molecular biology of cellulose degradation. Annu Rev Microbiol 44:219–248. doi:10.1146/annurev.mi.44.100190.001251

    Article  CAS  Google Scholar 

  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159. doi:10.1016/0003-2697(87)90021-2

    Article  CAS  Google Scholar 

  • Deshpande MV, Eriksson K, Pettersson LG (1984) An assay for selective detemination of exo-1, 4-β-glucanases in a mixture of celluloytic enzymes. Anal Biochem 138:481–487. doi:10.1016/0003-2697(84)90843-1

    Article  CAS  Google Scholar 

  • Fritscher C, Messner R, Kubicek CP (1990) Cellobiose metabolism and cellobiohydrolase I biosynthesis by Trichoderma reesei. Exp Mycol 14:405–415. doi:10.1016/0147-5975(90)90063-Y

    Article  CAS  Google Scholar 

  • Ilmén M, Saloheimo A, Onnela M et al (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298–1306

    Google Scholar 

  • Kalra MK, Sidhu MS, DK Sandhu et al (1984) Production and analysis of the cellulases in Trichoderma reesei. Appl Microbiol Biotechnol 20:427–429. doi:10.1007/BF00261948

    Article  CAS  Google Scholar 

  • Kawamori M, Morikawa Y, Talasawa S (1985) Inductive formation of cellulase by L-sorbose in Trichoderma reesei. Appl Microbiol Biotechnol 22:235–236. doi:10.1007/BF00253616

    Article  CAS  Google Scholar 

  • Mandels M, Reese ET (1960) Induction of cellulase in fungi by cellobiose. J Bacteriol 79:816–826

    CAS  Google Scholar 

  • Morikawa Y, Ohashi T, Mantani O et al (1995) Cellulase induction by lactose in Trichoderma reesei PC-37. Appl Microbiol Biotechnol 44:106–111. doi:10.1007/BF00164488

    Article  CAS  Google Scholar 

  • Seiboth B, Hakola S, Mach RL et al (1997) Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei. J Bacteriol 179:5318–5320

    CAS  Google Scholar 

  • Stricker AR, Grosstessner-Hain K, Würleitner E et al (2006) Xyr1 (xylanase regulator 1) regulates both the hydrolytic enzyme system and D-xylose metabolism in Hypocrea jecorina. Eukaryot Cell 5:2128–2137. doi:10.1128/EC.00211-06

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by grants from the National Natural Science Foundation of China (20066001) and the Open Fund of Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization (SB0709).

Author information

Authors and Affiliations

  1. College of Life Science and Technology, Guangxi University, 530004, Nanning, People’s Republic of China

    Min Ling, Guiguang Chen, Yuanshan Lin & Zhiqun Liang

  2. Department of Biochemistry and Molecular Biology, Guangxi Medical University, 530021, Nanning, People’s Republic of China

    Min Ling

Authors
  1. Min Ling
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guiguang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuanshan Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhiqun Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhiqun Liang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ling, M., Chen, G., Lin, Y. et al. Induction of cellulase gene transcription by a novel oligosaccharide: molasses alcohol stillage substance. World J Microbiol Biotechnol 25, 1485–1489 (2009). https://doi.org/10.1007/s11274-009-0017-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-009-0017-3

Keywords

Search

Navigation