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Exiguobacterium acetylicum strain 1P (MTCC 8707) a novel bacterial antagonist from the North Western Indian Himalayas

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Abstract

Exiguobacterium acetylicum strain 1P (MTCC 8707) is a rhizospheric, Gram positive, rod shaped, yellow pigmented bacterium isolated from an apple orchard rhizospheric soil, on nutrient agar plates incubated at 4°C. The species level identification was arrived on the basis of 16S rRNA gene sequencing. The sequence showed 98% similarity with sequences of E. acetylicum available in the public domain. The strain was positive for siderophore and HCN production. In separate invitro assays it was found to inhibit the growth and development of Rhizoctoniasolani, Sclerotium rolfsii, Pythium and Fusarium oxysporum. The volatile compound produced by the bacterium was found to be the most potent in inhibiting the hyphal development of R. solani, S. rolfsii, Pythium and F. oxysporum by 45.55, 41.38, 28.92 and 39.74% respectively. Commonly observed deformities caused by the diffusible and volatile compounds produced by the bacterium included hyphal inhibition, constriction and deformation. Under pot culture conditions the bacterium improved the germination and early growth parameters of pea (Pisum sativum) in the presence of R. solani and S. rolfsii.

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Acknowledgments

This work was supported by the Indian Council of Agricultural Research, through the project “Application of Microorganisms in Agriculture and Allied Sectors”. Dr. Anita Pandey, GBPIHED, Almora and Dr. Deeksha Joshi, VIHA, Almora, are acknowledged for providing the plant pathogenic fungal cultures used in this study. Sequencing of the 16S rRNA gene was performed at the Institute of Microbial Technology, Chandigarh, India.

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  1. Vivekananda Institute of Hill Agriculture (Indian Council of Agricultural Research), Almora, Uttarakhand, 263601, India

    Govindan Selvakumar, Piyush Joshi, Sehar Nazim, Pankaj K. Mishra, Samaresh Kundu & Hari S. Gupta

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  1. Govindan Selvakumar
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  2. Piyush Joshi
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  3. Sehar Nazim
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  4. Pankaj K. Mishra
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  5. Samaresh Kundu
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  6. Hari S. Gupta
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Correspondence to Govindan Selvakumar.

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Selvakumar, G., Joshi, P., Nazim, S. et al. Exiguobacterium acetylicum strain 1P (MTCC 8707) a novel bacterial antagonist from the North Western Indian Himalayas. World J Microbiol Biotechnol 25, 131–137 (2009). https://doi.org/10.1007/s11274-008-9874-4

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  • DOI: https://doi.org/10.1007/s11274-008-9874-4

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