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Amelioration of phytotoxic effects of Cd on mung bean seedlings by gluconic acid secreting rhizobacterium Enterobacter asburiae PSI3 and implication of role of organic acid

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Abstract

Mung bean seedlings inoculated with Enterobacter asburiae PSI3, a gluconic acid-producing rhizosphere isolate, enhanced plant growth in the presence of phytotoxic levels of Cd2+ in gnotobiotic pot experiments as compared to the uninoculated Cd-treated plants. Addition of organic acids to Cd-stressed seedlings promoted root elongation. Hematoxylin competition assays showed that organic acids could displace Cd2+ from the Cd2+: hematoxylin complex in the same order of effectiveness as was found for restoration of root net elongation viz. oxalate > malate > succinate while gluconate was effective at higher concentrations. Root associated Cd2+, assessed by hematoxylin staining of roots was found to be reduced when roots were treated with organic acid. Cd stress increased antioxidant enzymes such as peroxidase and superoxide dismutase in mung bean roots while organic acid treatment suppressed the up-regulation of these enzymes by Cd.

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Acknowledgments

S. Shukla is grateful to Council of Scientific and Industrial Research (CSIR), New Delhi, India for providing fellowship.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology Center, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, 390002, Gujarat, India

    B. Kavita, Sapna Shukla & G. Archana

  2. Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, 390002, Gujarat, India

    G. Naresh Kumar

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  1. B. Kavita
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  4. G. Archana
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Correspondence to G. Archana.

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Kavita, B., Shukla, S., Naresh Kumar, G. et al. Amelioration of phytotoxic effects of Cd on mung bean seedlings by gluconic acid secreting rhizobacterium Enterobacter asburiae PSI3 and implication of role of organic acid. World J Microbiol Biotechnol 24, 2965–2972 (2008). https://doi.org/10.1007/s11274-008-9838-8

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