Skip to main content
SpringerLink
Log in

Insecticidal action of Quinomycin A from Streptomyces sp. KN-0647, isolated from a forest soil

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

An actinomycete strain KN-0647 was isolated from a forest soil sample collected from Dali Cangshan mountain, Yunnan Province, China. The strain was identified as Streptomyces sp. according to the morphological, physiological characteristics and whole nucleotide sequence analysis of 16S rRNA gene, and could not be identified up to species level, just suggesting a potential new taxon. The ethyl acetate extract from this strain displayed growth inhibition on the test pathogenetic insects, such as Spodoptera exigua, Dendrolimus punctatus, Plutella xylostella, Aphis glycines and Culex pipiens. The active compound was isolated and identified through a combination of spectral and chemical methods (UR, MS, and 1HNMR) as quinomycin A. This is the first report on the insecticidal activity of antibiotic quinomycin A.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Arai T (1976) Actinomycetes; The Boundary Microorganisms. Toppan, Singapore

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–789

    Article  Google Scholar 

  • Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    Article  CAS  Google Scholar 

  • Kelly KL (1964) Inter-society color council—National bureau of standards color name charts illustrated with centroid colors. Washington, DC: US Government Printing Office

    Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16:111–120

    Article  CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  CAS  Google Scholar 

  • Li GK, Zhang Q, Jiang Y, Zhu BQ, Hu HF (2004) Studies on compounds SIPI-1140A and B with inhibitory activity against antibiotic-resistant microbes from microorganisms . Chin J Nat Med 12 (3):189–192

    Google Scholar 

  • Li WJ, Xu P, Schumann P, Zhang YQ, Pukall R, Xu LH, Stackebrandt E, Jiang CL (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  Google Scholar 

  • Locci R (1989) Streptomyces and related genera. In: Williams ST, Sharpe ME, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins Co, Baltimore, pp 2463–2468

    Google Scholar 

  • Martin DG, Mizsak SA, Biles C (1975) Structure of quinomycin antibiotics. J Antibiot 28 (4):332–339

    CAS  Google Scholar 

  • NIAID (2001) NIAID Global Health Research Plan for HIV/AIDS, Malaria and Tuberculosis US, Department of Health and Human Services, Bethesda, MD

  • Pridham TG, Lyons AJ (1980) Methodologies for Actinomycetales with special reference to streptomycetes and streptoverticillia. In Dietz A, Thayer DW (eds) Actinomycete Taxonomy, Special publication no 6. Arlington, VA: Society for Industrial Microbiology, pp 153–224

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tree. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Searle MS (1994) Binding of quinomycin antibiotic UK-65,662 to DNA: 1H-nmr studies of drug-induced changes in DNA conformation in complexes with d (ACGT)2 and d(GACGTC)2. Biochem J 304:967–979

    CAS  Google Scholar 

  • Shi JJ, Qi CQ, Chen WJ (1999) The revision of 1H, 13C-NMR assignments of quinomycin A and C. J Chin Antibiot 24(4):258–262

    CAS  Google Scholar 

  • Shirling EB, Gottlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  • Siddiqui BS, Afshan F, Ghiasuddin, Faizi S, Naqvi SNH, Tariq RM (2000) Two insecticidal tetranortriterpenoids from Azadirachta indica. Phytochemistry 53:371–376

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  • Ward D, Reich E, Goldberg IH (1965) Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs. Science 149:1259–1263

    Article  CAS  Google Scholar 

  • Zin NM, Sarmin NM, Ghadin N, Basri DF, Sidik NK, Hess WM, Strobel GA (2007) Bioactive endophytic streptomycetes fromtheMalay Peninsula. FEMS Microbiol Lett 274:83–88

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by National Significant Basic Project of China (973 2003CB114201 and 2007CB109203). We also thank Dr. Mariko (Meiji Seika Kaisha, Ltd. Agricultural & Veterinary Research Labs Biology Group) for the analysis of chemical structure. W.-J. Li was supported by Program for New Century Excellent Talents in University.

Author information

Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100094, P.R. China

    Huamei Liu & Jie Zhang

  2. National Enterprise Technology Centre, Wuhan Kernel Biopesticide Co., Ltd, Guannan Industry Zone, Wuhan, 430072, P.R. China

    Huamei Liu

  3. The Key Laboratory for Microbial Resources, Ministry of Education, Kunming, P.R. China

    Sheng Qin, Yongxia Wang & Wenjun Li

  4. Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan, 650091, P.R. China

    Sheng Qin, Yongxia Wang & Wenjun Li

Authors
  1. Huamei Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sheng Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yongxia Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wenjun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jie Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jie Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, H., Qin, S., Wang, Y. et al. Insecticidal action of Quinomycin A from Streptomyces sp. KN-0647, isolated from a forest soil. World J Microbiol Biotechnol 24, 2243–2248 (2008). https://doi.org/10.1007/s11274-008-9736-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-008-9736-0

Keywords

Search

Navigation