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Molecular and phenotypic characterization of potential plant growth-promoting Pseudomonas from rice and maize rhizospheres

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Abstract

In this study, Pseudomonas species were isolated from the rhizospheres of two plant hosts: rice (Oryza sativa cultivar Pathum Thani 1) and maize (Zea mays cultivar DK888). The genotypic diversity of isolates was determined on basis of amplified rDNA restriction analysis (ARDRA). This analysis showed that both plant varieties selected for two distinct populations of Pseudomonas. The actual biocontrol and plant promotion abilities of these strains was confirmed by bioassays on fungal (Verticillum sp., Rhizoctonia solani and Fusarium sp.) and bacterial (Ralstonia solanacearum and Bacillus subtilis) plant pathogens, as well as indole-3-acetic acid (IAA) production and carbon source utilization. There was a significant difference between isolates from rice and maize rhizosphere in terms of biological control against R.  solanacearum and B.  subtilis. Interestingly, none of the pseudomonads isolated from maize rhizosphere showed antagonistic activity against R.  solanacearum. This study indicated that the percentage of pseudomonad isolates obtained from rice rhizosphere which showed the ability to produce fluorescent pigments was almost threefold higher than pseudomonad isolates obtained from maize rhizosphere. Furthermore, the biocontrol assay results indicated that pseudomonad isolated from rice showed a higher ability to control bacterial and fungal root pathogens than pseudomonad isolates obtained from maize. This work clearly identified a number of isolates with potential for use as plant growth-promoting and biocontrol agents on rice and maize.

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Acknowledgements

This work was supported by a grant from the Royal Golden Jubilee Ph.D. program (Thailand Research Fund) and Suranaree University of Technology. F.O. was supported in part by grants awarded by Science Foundation Ireland (SFI 02/IN.1/B1261 and 04/BR/B0597 to F.O.) and the Health Research Board (RP/2004/145 and RP/2006/271 to F.O.).

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Authors and Affiliations

  1. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

    Phrueksa Lawongsa, Nantakorn Boonkerd & Neung Teaumroong

  2. School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand

    Sopone Wongkaew

  3. Department of Microbiology, Biomerit Research Centre, National University of Ireland, Cork, Ireland

    Fergal O’Gara

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  1. Phrueksa Lawongsa
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  2. Nantakorn Boonkerd
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  3. Sopone Wongkaew
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  4. Fergal O’Gara
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  5. Neung Teaumroong
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Correspondence to Neung Teaumroong.

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Lawongsa, P., Boonkerd, N., Wongkaew, S. et al. Molecular and phenotypic characterization of potential plant growth-promoting Pseudomonas from rice and maize rhizospheres. World J Microbiol Biotechnol 24, 1877–1884 (2008). https://doi.org/10.1007/s11274-008-9685-7

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  • DOI: https://doi.org/10.1007/s11274-008-9685-7

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