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Phenazine-1-carboxylic acid biosynthesis in Pseudomonas Chlororaphis GP72 is positively regulated by the sigma factor RpoN

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Abstract

The production of phenazine-1-carboxylic acid (PCA) and 2-hydroxyphenazine (2-OH-PHZ) makes Pseudomonas chlororaphis GP72 an effective biocontrol agent. In order to understand how production of PCA is regulated by RpoN, an insertional mutation in rpoN has been made in P. chlororaphis GP72. Production of PCA in the rpoN mutant strain GP72N decreased both in King’s B medium and in Pigment Producing Medium. Moreover, the expression of the translational fusion phzA´–´lacZ was reduced about 2-fold in GP72N compared to wild type strain, whatever the growth medium is. Complementation of rpoN gene in mutant GP72N restored its motility and its PCA biosynthesis ability. However, overexpression of RpoN had no major effects on the expression of the RpoN-dependent phenotypes described in this study for P. chlororaphis GP72. These results suggest that RpoN is involved as a positive regulator in the regulation of PCA biosynthesis in P. chlororaphis GP72.

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References

  • Alarcón-Chaidez FJ, Bender CL (2001) Analysis of the rpoN locus in the plant pathogenic bacterium Pseudomonas syringae pv. glycinea. DNA Seq 12:77–84

    Article  Google Scholar 

  • Barrios H, Valderrama B, Morett E (1999) Compilation and analysis of σ54-dependent promoter sequences. Nucleic Acids Res 72:4305–4313

    Article  Google Scholar 

  • Buck M, Gallegos MT, Studholme DJ, Guo YL, Gralla JD (2000) The bacterial enhancer-dependent σ54 (σN) transcription factor. J Bacteriol 182:4129–4136

    Article  CAS  Google Scholar 

  • Girard G, Van Rij ET, Lugtenberg BJJ, Bloemberg GV (2006) Regulatory roles of psrA and rpoS in phenazine-1-carboxamide synthesis by Pseudomonas chlororaphis PCL1391. Microbiology 152:43–58

    Article  CAS  Google Scholar 

  • Haas D, Défago G (2005) Biological control of soil-borne pathogens by fluorescent pseudomonads. Nat Rev Microbiol 3:309–319

    Article  Google Scholar 

  • Handelsman J, Stabb EV (1996) Biocontrol of soilborne plant pathogens. Plant Cell 8:1855–1869

    Article  CAS  Google Scholar 

  • Härtig E, Zumft WG (1998) The requirement of RpoN (sigma factor σ54) in denitrification by Pseudomonas stutzeri is indirect and restricted to the reduction of nitrite and nitric oxide. Appl Environ Microbiol 64:3092–3095

    Google Scholar 

  • Heeb S, Blumer C, Haas D (2002) Regulatory RNA as mediator in GacA/RsmA-dependent global control of exoproduct formation in Pseudomonas fluorescens CHA0. J Bacteriol 184:1046–1056

    Article  CAS  Google Scholar 

  • Hoang TT, Karkhoff-Schweizer RR, Kutchma AJ, Schweizer HP (1998) A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:103–119

    Article  Google Scholar 

  • Huang XQ, Yan A, Zhang XH, Xu YQ (2006) Identification and characterization of a putative ABC transporter PltHIJKN required for pyoluteorin production in Pseudomonas sp. M18. Gene 376:68–78

    Article  CAS  Google Scholar 

  • Jin S, Ishimoto K, Lory S (1994) Nucleotide sequence of the rpoN gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa. J Bacteriol 176:1316–1322

    CAS  Google Scholar 

  • Jurado P, Fernandez LA, De Lorenzo V (2003) Sigma 54 levels and physiological control of the Pseudomonas putida Pu promoter. J Bacteriol 185:3379–3383

    Article  CAS  Google Scholar 

  • King EO, Ward MK, Raney DE (1954) Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307

    CAS  Google Scholar 

  • Kluyver AJ (1956) Pseudomonas aureofaciens nov. spec. and its pigments. J Bacteriol 72:406–411

    CAS  Google Scholar 

  • Köhler T, Harayama S, Ramos JL, Timmis KN (1989) Involvement of Pseudomonas putida RpoN sigma factor in regulation of various metabolic functions. J Bacteriol 171:4326–4333

    Google Scholar 

  • Liu HM, He YJ, Jiang HX, Peng HS, Huang XQ, Zhang XH, Thomashow LS, Xu YQ (2007) Characterization of a phenazine-producing strain Pseudomonas chlororaphis GP72 with broad-spectrum antifungal activity from green pepper rhizosphere. Curr Microbiol 54(4):302–306

    Article  CAS  Google Scholar 

  • Lukashin AV, Borodovsky M (1998) GeneMark.hmm: new solutions for gene finding. Nucleic Acids Res 26:1107–1115

    Article  CAS  Google Scholar 

  • Maurhofer M, Reimmann C, Schmidli-Sacherer P, Heeb S, Haas D, Défago G (1998) Salicylic acid biosynthetic genes expressed in Pseudomonas fluorescens strain P3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus. Phytopathology 88:678–684

    Article  CAS  Google Scholar 

  • Merrick MJ (1993) In a class of its own—the RNA polymerase sigma factor σ54 (σN). Mol Microbiol 10:903–909

    Article  CAS  Google Scholar 

  • Michiels J, Van Soom T, D’Hooghe I, Dombrecht B, Benhassine T, De Wilde P, Vanderleyden J (1998) The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN, and ptsA mutants. J Bacteriol 180:1729–1740

    CAS  Google Scholar 

  • Reese MG (2001) Application of a time-delay neutral network to promoter annotation in the Drosophila melanogaster genome. Comput Chem 26:51–56

    Article  CAS  Google Scholar 

  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Sprong Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  • Schweizer HD (1993) Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. Biotechniques 15:831–834

    CAS  Google Scholar 

  • Zhu DH, Xu WJ, Geng HF, Zhang XH, Xu YQ (2003) Gene cloning of rpoD and its impact on biosynthesis of antibiotics in Fluorescent pseudomonas M18. Acta Microbiologica Sinica 43(3):315–323

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Key Laboratory of Microbial Metabolism, Ministry of Education and College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, P.R. China

    Hai-Ming Liu, An Yan, Xue-Hong Zhang & Yu-Quan Xu

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  1. Hai-Ming Liu
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  2. An Yan
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  3. Xue-Hong Zhang
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  4. Yu-Quan Xu
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Correspondence to Yu-Quan Xu.

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Liu, HM., Yan, A., Zhang, XH. et al. Phenazine-1-carboxylic acid biosynthesis in Pseudomonas Chlororaphis GP72 is positively regulated by the sigma factor RpoN. World J Microbiol Biotechnol 24, 1961–1966 (2008). https://doi.org/10.1007/s11274-008-9655-0

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  • DOI: https://doi.org/10.1007/s11274-008-9655-0

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