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Characterization of a Phenazine-Producing Strain Pseudomonas chlororaphis GP72 with Broad-Spectrum Antifungal Activity from Green Pepper Rhizosphere

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Abstract

A new Pseudomonas strain, designated GP72, was isolated from green pepper rhizosphere and identified as a member of species Pseudomonas chlororaphis based on morphology; conventional biochemical and physiologic tests; Biolog GN system (Biolog Inc., Hayward, CA); and 16S rDNA sequence analysis. The secondary metabolites produced by this strain have shown broad-spectrum antifungal activity against various phytopathogens of agricultural importance in vitro. Two main antifungal substances produced by this strain proved to be phenazine-1-carboxylic acid and 2-hydroxyphenazine with further purification and structure elucidation based on ultraviolet-absorbent spectrum scanning, atmospheric pressure chemical ionization–mass spectrometry (APCI-MS) spectrum, and 1H,13C nuclear magnetic resonance spectrums. Strain GP72 could produce quorum-sensing signaling molecules of N-butanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone, which were found to accumulate with different quantities in King’s medium B and pigment producing medium, respectively.

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Acknowledgments

We gratefully thank Dieter Haas for generously providing the plasmids and strains used in this study. This work was financially supported by Grant No. 2004BA308A02-14 from the 10th Five-Year Programs of Chinese National Science and Technology Development and Grant No. 30370041 from the National Science Foundation.

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Authors and Affiliations

  1. Key Laboratory of Microbial Metabolism, Ministry of Education and College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, PRC

    Haiming Liu, Yanjing He, Haixia Jiang, Huasong Peng, Xianqing Huang, Xuehong Zhang & Yuquan Xu

  2. Root Disease and Biologic Control Research Unit, United States Department of Agriculture Agricultural Research Service, Pullman, WA, 99164-6430, USA

    Linda S. Thomashow

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  1. Haiming Liu
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  2. Yanjing He
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  3. Haixia Jiang
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  4. Huasong Peng
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  5. Xianqing Huang
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  6. Xuehong Zhang
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  7. Linda S. Thomashow
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  8. Yuquan Xu
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Correspondence to Yuquan Xu.

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Liu, H., He, Y., Jiang, H. et al. Characterization of a Phenazine-Producing Strain Pseudomonas chlororaphis GP72 with Broad-Spectrum Antifungal Activity from Green Pepper Rhizosphere. Curr Microbiol 54, 302–306 (2007). https://doi.org/10.1007/s00284-006-0444-4

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  • DOI: https://doi.org/10.1007/s00284-006-0444-4

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