Abstract
Thirteen thermophilic and thermotolerant fungal cultures isolated from composting soils produced diverse β-glucosidases as indicated by zymograms of PAGE developed using 4-methylumbelliferyl-β-d-glucoside. IEF profiling revealed the presence of 28 β-glucosidases separated on the basis of their pI. Eleven of the β-glucosidases were active under acidic conditions. Two β-glucosidase isoforms, ASCβG-II of Aspergillus caespitosus and HIβG-I of Humicola insolens were resistant to inhibition by glucose and were active in the presence of 300 and 100 (mM) glucose, respectively.
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Financial support provided by Department of Biotechnology, New Delhi (India) for carrying out this study is duly acknowledged.
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Sonia, K.G., Chadha, B.S., Badhan, A.K. et al. Identification of glucose tolerant acid active β-glucosidases from thermophilic and thermotolerant fungi. World J Microbiol Biotechnol 24, 599–604 (2008). https://doi.org/10.1007/s11274-007-9512-6
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DOI: https://doi.org/10.1007/s11274-007-9512-6