Abstract
Physiological regulation of laccase production from Ganoderma sp. KU-Alk4, isolated in Thailand, was controlled by the initial glucose concentration in liquid culture. Different laccase isozymes were produced using different starting concentrations of glucose. With 1% glucose, two isozymes, KULac 1 and 2 were produced, while with 4% glucose, three different isozymes, KULac 3, 4 and 5, were produced. The KULacs differed in their molecular mass, ranging from 53 to 112 kDa. KULac 2 was a new laccase that had a different N-terminal amino acid sequence from other laccases previously reported. All the isozymes had optimum pH at 3.5 and were stable over the wide range of pH, 3.0–10.0, especially in alkaline pH. It is noteworthy that the activities of the four KULacs with 2,6-dimethoxyphenol were extremely high up to 90°C. They retained 100% of their activities at 60°C for 1 h.
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Acknowledgments
We are grateful to The Royal Golden Jubilee Ph.D. Program of the Thailand Research fund for the support to CT. We thank Graduate School of Agricultural Science, Tohoku University, Japan for N-terminal analysis facility in particular Prof. Yoshiyuki Kamio, Laboratory of Applied Microbiology, for host and JSPS-NRCT for funding to LC for her short visit. Thanks to Faculty of Science, Kasetsart University, for Sc-TRF fund.
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Teerapatsakul, C., Abe, N., Bucke, C. et al. Novel laccases of Ganoderma sp. KU-Alk4, regulated by different glucose concentration in alkaline media. World J Microbiol Biotechnol 23, 1559–1567 (2007). https://doi.org/10.1007/s11274-007-9401-z
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DOI: https://doi.org/10.1007/s11274-007-9401-z