Abstract
In this work, the heterotrophic cultivation of bacterium Paracoccus denitrificans has been studied in a horizontal rotating tubular bioreactor (HRTB). After development of a microbial biofilm on the inner surface of the HRTB, conditions for one-step removal of acetate and ammonium ion were created. The effect of bioreactor process parameters [medium inflow rate (F) and bioreactor rotation speed (n)] on the bioprocess dynamics in the HRTB was studied. Nitrite and nitrogen oxides (NO and N2O) were detected as intermediates of ammonium ion degradation. The biofilm thickness and the nitrite concentration were gradually reduced with increase of bioreactor rotation speed when the medium inflow rate was in the range of 0.5–1.5 l h−1. Further increase of inflow rate (2.0–2.5 l h−1) did not have a significant effect on the biofilm thickness and nitrite concentration along the HRTB. Complete acetate consumption was observed when the inflow rate was in the range of 0.5–1.5 l h−1 at all bioreactor rotation speeds. Significant pH gradient (cca 1 pH unit) along the HRTB was only observed at the highest inflow rate (2.5 l h−1). The results have clearly shown that acetate and ammonium ion removal by P. denitificans can be successfully conducted in a HRTB as a one-step process.
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References
Baker SC, Ferguson SJ, Ludwig B, Page MD, Richter OM, van Spanning RJ (1998) Molecular genetics of the genus Paracoccus: metabolically versatile bacteria with bioenergetic flexibility. Microbiol Mol Biol Rev 62:1046–1078
Baumann B, Snozzi M, Zehnder AJB, van der Meer JR (1996) Dynamics of denitrification activity of Paracoccus denitrificans in continuous culture during aerobic-anaerobic changes. J Bacterial. 178:4367–4374
Brading MG, Jass J, Lappin-Scott HM (1995) Dymanics of bacterial biofilm formation. In: Lappin-Scott HM, Costerton JW (eds.) Microbial biofilms, Cambridge University Press, Cambridge, pp 46–63
Characklis WG (1990) Biofilm processes. In: Characklis WG, Marshall KC (eds) Biofilms, Wiley, New York, pp 93–130
Cristian P, van Loosdrecht MCM, Heijnen JJ (2001) Two dimensional model of biofilm detachment caused by internal stress from liquid flow. Biotechnol Bioeng 72: 205–218
Crossman LC, Moir JWB, Enticknap JJ, Richardson DJ, Spiro S (1997) Heterologous expression of heterotrophic nitrification genes. Microbiology 143:3775–3783
Gavril M, Macoveanu M (2000) Attached-growth process engineering in wastewater treatment. Bioproc Eng 23:95–106
Gavrilescu M (2002) Engineering concerns and new developments in anaerobic wastewater treatment. Clean Techn Environ Policy 3:346–362
Geraats SGM, Hooijmans CM, van Niel EWJ, Robertson LA, Heijnen JJ, Luyben KChAM, Kuenen JG (1990) The use of metabolically structured model in the study of growth, nitrification and denitrification by Thiospheara pantotropha. Biotechnol Bioeng 36:921–930
Gupta AB, Gupta SK (2001) Simultaneous carbon and nitrogen removal from high strength domestic wastewater in an aerobic RBC biofilm. Wat Res 35:1714–1722
Hall-Stoodley L, Stoodley P (2002) Developmental regulation of microbial biofilms. Curr Opin Biotec 13:228–233
Hanegraaf PPF, Muller EB (2001) The dynamics of macromolecular composition of biomass. J Theor Biol 212: 237–251
Helmer C, Kunst S (1998) Simultaneous nitrification/denitrification in an aerobic biofilm system. Wat Sci Tech 37:183–187
Ivančić M, Šantek B, Novak S, Marić V (2004a) Fermentative bioconversion in a horizontal rotating tubular bioreactor. Proc Biochem 39:995–1000
Ivančić M, Šantek B, Novak S, Horvat P, Marić V (2004b) Bioprocess kinetics in a horizontal rotating tubular bioreactor. Bioproc Biosyst Eng 26:169–175
Kargi F, Eker S (2001) Comparison of the preformance of rotating perforated tubes and rotating biodisc biofilm reactors for wastewater treatment. Proc Biochem 37: 1201–1206
Liu Y, Tay J (2002) The essential role of hydrodynamic shear force in the formation of biofilm and granular sludge. Wat Res 36:1653–1665
Mayr B, Horvat P, Moser A (1992) Engineering approach to mixing quantification in bioreactors. Bioproc Eng 8:137–143
Moir JWB, Crossman LC, Spiro S, Richardson DJ (1996) The purification of ammonia monooxygenase from Paracoccus denitrificans. FEBS Lett 387:71–74
Moser A (1985) Imperfectly mixed bioreactor systems. In: Moo-Young M (ed) Comprehensive biotechnology, vol. 2, Pergamon Press, Oxford, pp 77–98
Moser A (1988) Bioprocess technology kinetics and reactors. Springer, Wien New York
Neu TR, Lawrence JR (1997) Development and structure of microbial biofilms in river water studied by co-focal laser scanning microscopy. FEMS Microbiol Ecolol 24:11–25
Nicolella C, Loosdrecht MCM, Heijnen JJ (2000) Wastewater treatment with particulate biofilm reactor. J Biotechnol 80:1–33
Patricia AMA, Robertson LA, Kuenen JG (1995) Nitrification and denitrification by Thiosphaera pantotropha in aerobic chemostat cultures. FEMS Microbiol Ecolog 18:305–315
Peyton BM (1996) Effects of shear stress and substrate loading rate on Pseudomonas aeruginosa biofilm thickness and density. Wat Res 30:29–36
Peyton BM, Characklis WG (1993) A statistical analysis of the effect of substrate utilisation and shear stress on the kinetics of biofilm detachment. Biotechnol Bioeng 41:728–735
Robertson LA, Kuenen JG (1992) The effect of electron acceptor variations on the behaviour of Thiosphaera pantotropha and Paracoccus denitrificans in pure and mixed culture. FEMS Microbiol Ecol 86:221–228
Slavica A, Šantek B, Novak S, Marić V (2004) Microbial acetate oxidation in horizontal rotating tubular bioreactor. J Biosci 29: 169–177
Stewart PS (1993) A model of biofilm detachment. Biotechnol. Bioeng 41:111–117
Stoodley P, Hall-Stoodley L, Lappin-Scott HM (2000) Detachment, surface migration and other dynamic behavior in bacterial biofilms revealed by digital time-lapse imaging. Methods Enzymol 337:306–319
Šantek B, Horvat P, Novak S, Mayr B, Moser A, Marić V (1996a) Mathematical model of mixing in a horizontal rotating tubular bioreactor: simple flow model. Bioproc Eng 14:195–204
Šantek B, Horvat P, Novak S, Mayr B, Moser A, Marić V (1996b) Mathematical model of mixing in a horizontal rotating tubular bioreactor: spiral flow model. Bioproc Eng 14:223–229
Šantek B, Horvat P, Novak S, Moser A, Marić V (1998a) Studies of mixing in horizontal rotating tubular bioreactor: I. optimisation of adjustable model parameters for spiral flow model. Bioproc Eng 18:467–473
Šantek B, Horvat P, Novak S, Moser A, Marić V (1998b) Studies of mixing in horizontal rotating tubular bioreactor: II. prediction systems for adjustable parameters of spiral flow model. Bioproc Eng 19:19–28
Šantek B, Horvat P, Novak S, Moser A, Marić V (1998c) Studies of mixing in horizontal rotating tubular bioreactor: III. influence of liquid level and distance between the partition walls on prediction systems for adjustable model parameters. Bioproc Eng 19:91–102
Šantek B, Horvat P, Novak S, Sunko D, Moser A, Marić V (2000) Estimation of axial dispersion in horizontal rotating tubular bioreactor by means of a structured model. Bioproc Eng 23:265–274
Thomsen JK, Geest T, Cox RP (1994) Mass spectrometric studies of the effect of pH on the accumulation of intermediates in denitrification by Paracoccus denitrificans. Appl Environ Microbiol 60:536–541
U.S. EPA 40CFR60-test methods: method 7-Determination of nitrogen oxide emissions from stationary sources 1997, pp 1–12. Available at http://www.arb.ca.gov/testmeth/vol1/m_7.pdf
Venkataraman R, Ramanujam TK (1998) A study of microbiology of biological film layer in rotating biological contactors. Bioproc Eng 18:181–186
Zumft WG (1997) Cell biology and molecular basis of denitrification. Microbiol Mol Biol Rev 61:533–616
Yoo K, Ahn KH, Lee HJ, Kwak YI, Song KG (1999) Nitrogen removal from synthetic wastewater by simultaneous nitrification and denitification (SND) via nitrite in an intermittently-aerated reactor. Wat Res 33:145–154
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Rezić, T., Šantek, B., Novak, S. et al. Heterotrophic cultivation of Paracoccus denitrificans in a horizontal rotating tubular bioreactor. World J Microbiol Biotechnol 23, 987–996 (2007). https://doi.org/10.1007/s11274-006-9324-0
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DOI: https://doi.org/10.1007/s11274-006-9324-0