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Enhanced production of laccase activity by Trametes versicolor immobilized into alginate beads by the addition of different inducers

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Abstract

In the present paper the effect of adding veratryl alcohol and copper sulphate on laccase activity production by Trametes versicolor immobilized into alginate beads has been investigated. Employing copper sulphate as laccase inducer or supplementing the culture medium with veratryl alcohol, led to maximum values of laccase activity. However, the highest laccase activity (around 4,000 U l−1) was obtained in cultures simultaneously supplemented with copper sulphate (3 mM) and veratryl alcohol (20 mM). These values implied a considerable enhancement in relation to␣control cultures without any inducer (around 200 U l−1).

The production of laccase by immobilized T. versicolor in a 2-l airlift bioreactor with the optimized inducer has been evaluated. Laccase activities around 1,500 U l−1 were attained. The bioreactor operated for 44 days without operational problems and the bioparticles (fungus grows in alginate beads) maintained their shape throughout the fermentation. Moreover, the extracellular liquid obtained was studied in terms of pH and temperature activity and stability. On the other hand, anthracene was added in two-repeated batches in order to determine the efficiency of this process to degrade pollutants. Near complete degradation was reached in both batches. Moreover, in vitro degradation of several polycyclic aromatic hydrocarbons by crude laccase was also performed.

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Acknowledgements

This research was financed by the Spanish Ministry of Science and Technology and European FEDER (Project CTM2004-01539/TECNO).

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Vigo, Campus Universitario As Lagoas-Marcosende, E-36310, Vigo, Spain

    Alberto Domínguez, Jose Gómez, Miriam Lorenzo & Ángeles Sanromán

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  1. Alberto Domínguez
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  2. Jose Gómez
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  3. Miriam Lorenzo
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  4. Ángeles Sanromán
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Correspondence to Ángeles Sanromán.

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Domínguez, A., Gómez, J., Lorenzo, M. et al. Enhanced production of laccase activity by Trametes versicolor immobilized into alginate beads by the addition of different inducers. World J Microbiol Biotechnol 23, 367–373 (2007). https://doi.org/10.1007/s11274-006-9233-2

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