Summary
New yeast isolates from unexplored Patagonian habitats were studied for the production of biomass and carotenoids as the first step towards the selection of hyper-producing strains and the design of a process optimization approach. Patagonian yeast isolates considered as potential biomass and carotenoid sources were studied using ammonium sulphate and urea as nitrogen sources in semi-synthetic medium (MMS), and agro-industrial byproducts (cane molasses, corn syrup, raw malt extract) as carbon sources. Maximum pigment production (300 μg g−1) was achieved by Rhodotorula mucilaginosa CRUB 0195 and by novel species Cryptococcus sp. CRUB 1046. β-carotene, torulene and torularhodin were the major carotenoids found.
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References
Bhosale P., Gadre R.V., 2001 beta-carotene production in sugarcane molasses by a Rhodotorula glutinis mutantJournal of Industrial Microbiology and Biotechnology 26: 327–332
Brizzio S., van Broock M.R., 1998 Characterization of wild yeast killer from Nahuel Huapi National Park (Patagonia, Argentina)Journal of Food Technology and Biotechnology 4: 273–278
Buzzini P., 2000 An optimization study of carotenoid production by Rhodotorula glutinis DBVPGG 3853 from substrates containing concentrated rectified grape must as the sole carbohydrate sourceJournal of Industrial Microbiology and Biotechnology 4: 41–45
Buzzini P., Martini A., 1999 Production of carotenoids by strains of Rhodotorula glutinis cultured in raw materials of agro-industrial origin Bioresource Technology 71: 41–44
Buzzini P., Rubinstein L., Martini A., 2001 Production of yeast carotenoids by using agro-industrial byproductsAgro-Food-Industry Hi-Tech 12: 7–10
Frengova G., Simova E., Pavlova K., Beshkova D., 1994 Formation of carotenoids by Rhodotorula glutinis in whey ultrafiltrateBiotechnology and Bioengineering 44: 888–894
Frengova G., Simova E., Beshkova D., 1995 Effect of temperature changes on the production of yeast pigments co-cultivated with lactic-acid bacteria in whey ultrafiltrateBiotechnology Letters 17: 1001–1006
Goodwin T.W., 1972 Carotenoids in fungi and non-photosynthetic bacteriaProgress in Industrial Microbiology 11: 29–88
Johnson E.A., Schroeder W.A., 1995 Microbial carotenoidsAdvances in Biochemical Engineering and Biotechnology 53: 121–178
Libkind D., Brizzio S., Ruffini A., Gadanho M., van Broock M., Sampaio J.P., 2003 Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia ArgentinaAntonie van Leeuwenhoek 84: 313–322
Libkind D., Brizzio S., van Broock M.R., 2004a Rhodotorula mucilaginosa, a carotenoid producing yeast strain from a Patagonian high altitude lakeFolia Microbiologica 49: 19–25
Libkind D., Pérez P., Sommaruga R., Diéguez M.C., Ferraro M., Brizzio S., Zagarese H., van Broock M.R., 2004b Constitutive and UV-inducible synthesis of photoprotective compounds (carotenoids and mycosporines) by freshwater yeastsPhotochemical and Photobiologial Sciences 3: 281–286
Libkind D., Gadanho M., van Broock M.R., Sampaio J.P., 2005 Sporidiobolus longiusculus sp. nov. and Sporobolomyces patagonicus sp. nov., novel yeasts of the Sporidiobolales isolated from aquatic environments in Patagonia, ArgentinaInternational Journal of Systematic and Evolutionary Microbiology 55: 503–509
Loehr R.C., 1974 Waste characteristics In Loehr RC, ed. Agricultural Waste Management. Problems, Processes and Approaches Academic Press London pp 100–126, ISBN 0-12-455250-1
Nelis H.J., De Leenheer A.P., 1991 Microbial sources of carotenoid pigments used in foods and feedsJournal of Applied Bacteriology 70: 181–191
Perrier V., Dubreucq E., Galzy P., 1995 Fatty acid and carotenoid composition of Rhodotorula strainsArchives of Microbiology 164: 173–179
Sakaki H., Nochide H., Nakanishi T., Miki W., Fujita T., Komemushi S., 1999 Effect of culture condition on the biosynthesis of carotenoids in Rhodotorula glutinis No. 21Seibutsu Kogaku Kaishi 77: 55–59
Shih C., Hang Y., 1995 Production of carotenoids by Rhodotorula rubra from sauerkraut brineLebensmittelwissenchaft und -Technologie 29: 570–572
Simpson K.L., Nakayama T.O.M., Chichester C.O., 1964 Biosynthesis of yeast carotenoidsJournal of Bacteriology 88: 1688–1694
Somashekar D., Joseph R., 2000 Inverse relationship between carotenoid and lipid formation in Rhodotorula gracilis according to the C/N ratio of the growth mediumWorld Journal of Microbiology and Biotechnology 16: 491–493
Villafañe V.E., Helbling W.E., Zagarese H.E., 2001 Solar ultraviolet radiation and its impact on aquatic systems of Patagonia, South AmericaAmbio 30: 112–117
Acknowledgements
This work was financially supported by the Universidad Nacional del Comahue and CONICET Project B091 granted to MvB and by Biotay S. A. Special thanks are given to A. Caballero and G. Vobis, and to the laboratory of Fotobiología (CRUB) for providing TLC and spectrophotometer equipments for sample analysis.
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Libkind, D., van Broock, M. Biomass and carotenoid pigment production by patagonian native yeasts. World J Microbiol Biotechnol 22, 687–692 (2006). https://doi.org/10.1007/s11274-005-9091-3
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DOI: https://doi.org/10.1007/s11274-005-9091-3