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2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeast Saccharomyces cerevisiae

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Summary

Nikkomycin Z (NZ) is a competitive inhibitor of chitin synthase III in the yeast Saccharomyces cerevisiae. Myosin type II-deficient yeast strains (myo1) display a dramatic reduction in growth when chitin synthase III activity is inhibited by NZ, supporting the contention that actomyosin motility plays an important role in maintaining cell wall integrity. A proposed inhibitor of cortical actin polymerization in vitro, 2,3-butanedione monoxime (BDM), also inhibits growth of wild-type yeast strains at a concentration of 20 mM. In this study, we assayed for potential in vivo interplay between BDM-sensitive cell functions and cell wall chitin synthesis by testing for increased sensitivity to NZ during co-treatment with BDM at sub-inhibitory concentrations. Our results show that BDM can increase the sensitivity of yeast cells to Nikkomycin Z.

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Acknowledgements

We thank Laura M. Bretaña for editorial assistance. This research was supported by an NIH grant awarded to the University of Puerto Rico Medical Sciences Campus S06 GM08224 with partial support from grants G12 RR03051 and R25 GM61838.

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Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, University of Puerto Rico, P.O. Box 365067, 00936-5067, San Juan, Puerto Rico

    Félix E. Rivera-Molina, Sahily González-Crespo, Yasdet Maldonado-De la Cruz, José M. Ortiz-Betancourt & José R. Rodríguez-Medina

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  1. Félix E. Rivera-Molina
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  2. Sahily González-Crespo
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  3. Yasdet Maldonado-De la Cruz
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  4. José M. Ortiz-Betancourt
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  5. José R. Rodríguez-Medina
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Correspondence to José R. Rodríguez-Medina.

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Rivera-Molina, F.E., González-Crespo, S., Cruz, Y.MD.l. et al. 2,3-Butanedione monoxime increases sensitivity to Nikkomycin Z in the budding yeast Saccharomyces cerevisiae . World J Microbiol Biotechnol 22, 255–260 (2006). https://doi.org/10.1007/s11274-005-9028-x

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  • DOI: https://doi.org/10.1007/s11274-005-9028-x

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