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Decolorization of 1:2 metal complex dye Acid blue 193 by a newly isolated fungus, Cladosporium cladosporioides

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Summary

A fungus Cladosporium cladosporioides isolated from coal sample as a decolorizing microorganism. It decolorized five different azo and triphenylmethane dyes like acid blue 193, acid black 210, crystal violet, reactive black B(S) and reactive black BL/LPR both on solid and in liquid broth medium. Culture broth of this fungus decolorized completely 100 mg of acid blue 193 l−1 in 8 days. The extracellular enzyme of Cladosporium cladosporioides decolorized acid blue 193 on repeated addition to a total (out of 700 mg l−1) concentration of 564 mg l−1 within 168 h without significant decline in the activity, showing the resistant property of Cladosporium cladosporioides to a high concentration of the dye. The optimal temperature 40 °C, pH 5.6 and sugar concentration of 4% required for decolorization of acid blue 193. Cladosporium cladosporioides showed manganese peroxidase activity with 41 U l−1, laccase activity with 1413 U l−1 and lignin peroxidase activity was negligible after day 8 of incubation.

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Acknowledgements

The authors wish to thank the Department of Science and Technology (DST), New Delhi, India, for financial assistance through a research project SP/SO/BB-21/2003 dated 24.02.2004. We also wish to thank Prof. Narayan Reddy Department of Botany, Gulbarga University, Gulbarga for the identification of this fungus.

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Authors and Affiliations

  1. Department of Biochemistry, Gulbarga University, 585106, Gulbarga, Karnataka, India

    M.H. Vijaykumar, Y. Veeranagouda, K. Neelakanteshwar & T.B. Karegoudar

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  1. M.H. Vijaykumar
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  2. Y. Veeranagouda
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  3. K. Neelakanteshwar
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  4. T.B. Karegoudar
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Correspondence to T.B. Karegoudar.

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Vijaykumar, M., Veeranagouda, Y., Neelakanteshwar, K. et al. Decolorization of 1:2 metal complex dye Acid blue 193 by a newly isolated fungus, Cladosporium cladosporioides . World J Microbiol Biotechnol 22, 157–162 (2006). https://doi.org/10.1007/s11274-005-9013-4

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  • DOI: https://doi.org/10.1007/s11274-005-9013-4

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