Summary
Paecilomyces inflatus isolated from municipal waste compost was found to have cellulolytic activity in several solid and liquid media. This study was done to reveal the multifarious effects of municipal waste compost on endoglucanase activity of P. inflatus. The highest enzyme activities under the conditions of solid-state fermentation were measured in authentic compost samples compared with wood, straw and bran substrates. In surface liquid cultures glucose, cellobiose, xylan, Avicel cellulose, carboxymethylcellulose (CM-cellulose), starch and citrus pectin were used as carbon sources. All carbon sources supported the growth of P. inflatus. However, only CM-cellulose, cellobiose and pectin noticeably stimulated endoglucanase (EG) activity. Further stimulation of EG activity was obtained in cultures containing 1% CM-cellulose as a carbon source by supplementation with low-molecular mass aromatic compounds vanillin, veratric acid and benzoic acid, and with soil humic acid (SHA). SHA and veratric acid were found to be the most efficient elicitors of the cellulolytic activity. P. inflatus was able to utilize nitrate and ammonium as pure nitrogen sources in media containing cellulose.
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References
R.K. Adhikary P. Barua D.N. Bordoloi (1992) ArticleTitleInfluence of microbial pretreatment on degradation of mushroom nutrient substrate International Biodeterioration and Biodegradation 30 233–241 Occurrence Handle10.1016/0964-8305(92)90030-R
J.B. Almeida e Silva I.M. Mancilha M.C.D. Vanetti M.A. Teixeira (1995) ArticleTitleMicrobial protein production by Paecilomyces variotii cultivated on eucalyptus hemicellulosic hydrolyzate Bioresource Technology 52 197–200 Occurrence Handle10.1016/0960-8524(95)00029-E Occurrence Handle1:CAS:528:DyaK2MXntVGjtLo%3D
G. Almendros J. Dorado (1999) ArticleTitleMolecular characteristics related to the biodegradability of humic acid preparation European Journal of Soil Science 50 227–236 Occurrence Handle10.1046/j.1365-2389.1999.00240.x Occurrence Handle1:CAS:528:DyaK1MXktVyms70%3D
Aro, N., Pakula, T. & Penttilä, M. 2005 Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiology Reviews doi:10.1016/j.femsre.2004.11.006
M.J. Bailey K.M.H. Nevalainen (1981) ArticleTitleInduction, isolation and testing of stable Trichoderma reesei mutants with improved production of solubilizing cellulase Enzyme and Microbial Technology 3 153–157 Occurrence Handle10.1016/0141-0229(81)90076-4 Occurrence Handle1:CAS:528:DyaL3MXktVGju78%3D
P. Béguin J-P. Aubert (1994) ArticleTitleThe biological degradation of cellulose FEMS Microbiology Reviews 13 25–58
R.F.H. Dekker A-F.D. Vasconcelos A.M. Barbosa E.C. Giese L. Paccola-Meirelles (2001) ArticleTitleA new role for veratryl alcohol: regulation of synthesis lignocellulose-degrading enzymes in the ligninolytic ascomycetous fungus Botryosphaeria sp., influence of carbon source Biotechnology Letters 23 1987–1993 Occurrence Handle10.1023/A:1013742527550 Occurrence Handle1:CAS:528:DC%2BD38Xos1KjtQ%3D%3D
Domsch K.H., Gams W., Anderson T.H. 1980 Paecilomyces. In: Compendium of Soil Fungi. London: Academic Press, pp. 530–532. ISBN 0–12–220401–8
M.V. Dutton C.S. Evans (1996) ArticleTitleOxalate production by fungi: its role in pathogenicity and ecology in the soil environment Canadian Journal of Microbiology 42 881–895 Occurrence Handle1:CAS:528:DyaK28XmtFCgtLs%3D Occurrence Handle10.1139/m96-114
K-E. Eriksson B. Pettersson (1982) ArticleTitlePurification and partial characterization of two acidic proteases from the white-rot fungus Sporotrichum pulverulentum European Journal of Biochemistry 124 635–642 Occurrence Handle1:CAS:528:DyaL38XksFWlsrs%3D
F. Green SuffixIII T.A. Kuster T.L. Highley (1996) ArticleTitlePectin degradation during colonization of wood by brown-rot fungi Recent Research Developments in Plant Pathology 1 83–93
B. Henrissat H. Driguez C. Viet M. Schülein (1985) ArticleTitleSynergism of cellulases from Trichoderma reesei in the degradation of cellulose Biotechnology 3 722–726 Occurrence Handle1:CAS:528:DyaL2MXltVSrsb0%3D
T.L. Highley J.A. Micales (1990) ArticleTitleEffect of aromatic monomers on production of carbohydrate-degrading enzymes by white-rot and brown-rot fungi FEMS Microbiology Letters 66 15–22 Occurrence Handle1:CAS:528:DyaK3cXksFGksbY%3D
M. Hofrichter T. Vares M. Kalsi S. Galkin K. Scheibner W. Fritsche A. Hatakka (1999) ArticleTitleProduction of manganese peroxidase and organic acids and mineralization of 14C-labelled (14C-DHP) during solid-state fermentation of wheat straw with the white rot fungus Nematoloma frowardii Applied and Environmental Microbiology 65 1864–1870 Occurrence Handle1:CAS:528:DyaK1MXjtVanu7g%3D
A.C.P. Houston S. Visser R.A. Lautenschlager (1998) ArticleTitleResponse of microbial processes and fungal community structure to vegetation management in mixedwood forests soils Canadian Journal of Botany 76 2002–2010
R.L. Kainsa K.K. Kapoor M.M. Mishra (1979) ArticleTitleThe characterization of cellulase from Paecilomyces fusisporus Saksena Zentralblatt fr Bakteriologie 134 437–439 Occurrence Handle1:CAS:528:DyaE1MXlsFegtbc%3D
K.K. Kapoor M.K. Jain M.M. Mishra C.P. Singh (1978) ArticleTitleCellulase activity, degradation of cellulose and lignin and humus formation by cellulolytic fungi Annals of Microbiology 129 613–620
B. Kluczek-Turpeinen M. Tuomela A. Hatakka M. Hofrichter (2003) ArticleTitleLignin degradation in compost environment by the deuteromycete Paecilomyces inflatus Applied Microbiology and Biotechnology 61 374–379 Occurrence Handle1:CAS:528:DC%2BD3sXjs1GitLo%3D
Kluczek-Turpeinen, B., Steffen, K.T., Tuomela, M., Hatakka, A. & Hofrichter, M. 2005 Modification of humic acid by the compost-dwelling deuteromycete Paecilomyces inflatus.Applied Microbiology and Biotechnology 66, 443–449
C.P. Kubicek R. Messner F. Gruber M. Mandels E.M. Kubicek-Pranz (1993) ArticleTitleTriggering of cellulase biosynthesis by cellulose in Trichoderma reesei. Involvement of a constitutive, sophorose-inducible, glucose-inhibited beta-diglucoside permease Journal of Biological Chemistry 268 19364–19368 Occurrence Handle1:CAS:528:DyaK3sXltlGht7c%3D
M. Mes-Hartree C.M. Hogan J.N. Saddler (1988) ArticleTitleInfluence of growth substrate on production of cellulase enzymes by Trichoderma harzianum E85 Biotechnology and Bioengineering 31 725–729 Occurrence Handle10.1002/bit.260310715 Occurrence Handle1:CAS:528:DyaL1cXktVGhsLo%3D
M.M. Mishra C.P. Singh K.K. Kapoor M.K. Jain (1979) ArticleTitleDegradation of lignocellulosic material and humus formation fungi Annals of Microbiology 130 481–486
H.W. Müller W. Trösch K.D. Kulbe (1988) ArticleTitleEffect of phenolic compounds on the cellulose degradation by some white rot basidiomyctes FEMS Microbiology Letters 49 87–93 Occurrence Handle10.1111/j.1574-6968.1988.tb02687.x
J.C. Okolo S.K.C. Obi F.J.C. Odibo (1998) ArticleTitlePurification and characterization of two distinct carboxymethylcellulases of Paecilomyces sp Bioresource Technology 66 231–234 Occurrence Handle10.1016/S0960-8524(98)00053-4 Occurrence Handle1:CAS:528:DyaK1cXmsFOnu7Y%3D
J.I. Pitt A.D. Hocking (1999) Fungi and Food Spoilage Aspen Publishers Inc Gaithersburg Maryland 220–224
J. Rogalski A. Hatakka M. Wojtaś-Wasilewska A. Leonowicz (1993) ArticleTitleCellulolytic enzymes of the ligninolytic white-rot fungus Phlebia radiata Acta Biotechnologica 13 41–45 Occurrence Handle1:CAS:528:DyaK3sXksVCrtrg%3D
J. Ryckeboer J. Mergaert K. Vaes D. Klammer D. Clercq ParticleDe J. Coosemans H. Insam J. Swings (2003) ArticleTitleA survey of bacteria and fungi occurring during composting and self-heating processes Annals of Microbiology 53 349–410
R.A. Samson (1974) ArticleTitle Paecilomyces and some allied Hyphomycetes Studies in Mycology, Baarn 4 1–119
Z.A. Siddiqui I. Mahmood (1996) ArticleTitleBiological control of plant parasitic nematodes by fungi: a review Bioresource Technology 58 229–239 Occurrence Handle10.1016/S0960-8524(96)00122-8 Occurrence Handle1:CAS:528:DyaK2sXitV2isbs%3D
J.C. Steward J.B. Parry (1981) ArticleTitleFactor influencing the production of cellulase by Aspergillus fumigatus (Fresenius) Journal of General Microbiology 125 33–39
F.J. Stutzenberg A.J. Kayfman R.D. Lossin (1970) ArticleTitleCellulolytic activity in municipal solid waste composting Canadian Journal of Microbiology 16 553–560
Teeri, T.T. 1987 The cellulolytic enzyme system of Trichoderma reesei. Ph.D. thesis, Technical Research Centre of Finland (VTT), Finland
M. Tribak J.A. Ocampo I. Garcia-Romera (2002) ArticleTitleProduction of xyloglucanolytic enzymes by Trichoderma viride, Paecilomyces farinosus, Wardomyces inflatus and Pleurotus ostreatus Mycologia 94 404–410 Occurrence Handle1:CAS:528:DC%2BD38Xks1ens7k%3D
S. Tsujiyama (2003) ArticleTitleEffect of vanillin on the production of wood-decomposing enzymes from wood-rotting fungus, Coriolus versicolor Mycoscience 44 345–350 Occurrence Handle10.1007/s10267-003-0119-y Occurrence Handle1:CAS:528:DC%2BD3sXoslyhu78%3D
M. Tuomela A. Hatakka S. Raiskila M. Vikman M. Itävaara (2001) ArticleTitleBiodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in compost environment Applied Microbiology and Biotechnology 55 429–499 Occurrence Handle10.1007/s002530000513
M. Tuomela M. Vikman A. Hatakka M. Itävaara (2000) ArticleTitleBiodegradation of lignin in compost environment: a review Bioresource Technology 72 169–183 Occurrence Handle10.1016/S0960-8524(99)00104-2 Occurrence Handle1:CAS:528:DC%2BD3cXkt1Omtw%3D%3D
J. Varadi (1972) The effect of aromatic compounds on cellulase and xylanases production of the fungi Schizophyllum commune and Chaetomium globosum A.H. Walters E.H. Hueck-van Plas Particleder (Eds) Biodeterioration of Materials NumberInSeries2 Applied Science London 129–135
R.M. Vohra C.K. Shirkot S. Dhawan K.G. Gupta (1980) ArticleTitleEffect of lignin and some of its components on the production and activity of cellulase(s) by Trichoderma reesei Biotechnology and Bioengineering 22 1497–1500 Occurrence Handle10.1002/bit.260220714 Occurrence Handle1:CAS:528:DyaL3cXkvFemsrw%3D
T.M. Yazdi J.R. Woodward A. Radford (1990) ArticleTitleCellulase production by Neurospora crassa: The enzyme of the complex and their regulation Enzyme and Microbial Technology 12 116–119 Occurrence Handle1:CAS:528:DyaK3cXps12ntw%3D%3D
S.A. Waksman T.C. Cordon (1939) ArticleTitleThermophilic decomposition of plant residues in compost by pure and mixed cultures of microorganisms Soil Science 47 217 Occurrence Handle1:CAS:528:DyaA1MXjs1Smug%3D%3D
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Kluczek-Turpeinen, B., Maijala, P., Tuomela, M. et al. Endoglucanase Activity of Compost-Dwelling Fungus Paecilomyces inflatus is Stimulated by Humic Acids and Other Low Molecular Mass Aromatics. World J Microbiol Biotechnol 21, 1603–1609 (2005). https://doi.org/10.1007/s11274-005-8291-1
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DOI: https://doi.org/10.1007/s11274-005-8291-1