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Antifungal and Root Surface Colonization Properties of GFP-Tagged Paenibacillus brasilensis PB177

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Summary

This study evaluates the potential of Paenibacillus brasilensis strain PB177 to inhibit phytopathogenic fungi commonly causing maize diseases and to colonize maize plants. In vitro assays demonstrated antagonistic activity against the fungal pathogens, Fusarium moniliforme and Diplodia macrospora. The PB177 strain was tagged with the gfp gene, encoding the green fluorescent protein (GFP) and GFP-tagged bacteria were detected attached to maize roots by stereo- and confocal microscopy. The GFP-tagged bacteria were also used to treat maize seeds before challenging the seeds with two phytopathogenic fungi. The results demonstrated that the bacterial cells are mobilized to the maize roots in the presence of the fungal pathogens. The ability of P. brasilensis PB177 to inhibit fungal growth in vitro and its capability of colonization of maize roots in vivo suggest a potential application of this strain as a biological control agent. This is the first report on the successful introduction of the GFP marker gene into a P. brasilensis strain, enabling the direct observation of these promising plant growth promoting bacteria on maize roots in situ.

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Authors and Affiliations

  1. Section for Natural Sciences, Södertörn University College, Huddinge, Sweden

    I. von der Weid & J. K. Jansson

  2. Instituto de Microbiologia Prof. Paulo de Góes, Laboratório de Genética Microbiana, Departamento de Microbiologia Geral, Centro de Ciências da Saúde, , Universidade Federal do Rio de Janeiro, Bloco I, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brazil

    I. von der Weid & L. Seldin

  3. Department of Microbiology, Swedish University for Agricultural Sciences, Uppsala, Sweden

    V. Artursson & J. K. Jansson

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  1. I. von der Weid
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  2. V. Artursson
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  3. L. Seldin
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  4. J. K. Jansson
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Correspondence to L. Seldin.

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von der Weid, I., Artursson, V., Seldin, L. et al. Antifungal and Root Surface Colonization Properties of GFP-Tagged Paenibacillus brasilensis PB177. World J Microbiol Biotechnol 21, 1591–1597 (2005). https://doi.org/10.1007/s11274-005-8123-3

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  • DOI: https://doi.org/10.1007/s11274-005-8123-3

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