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Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media

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Summary

Few studies on screening of the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) have been reported. Here we present a simple potato-based medium to elaborate large quantities of extracellular PI-PLC from Bacillus thuringiensis serovar. kurstaki. The enzyme activity (1.75 U/ml) was found to be maximum in the potato-extract medium supplemented with sucrose and mineral salts with a specific activity of 3.88 U/mg protein.

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Authors and Affiliations

  1. Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, 641 006, Tamil Nadu, India

    T. Palvannan

  2. Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    R. Boopathy

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  1. T. Palvannan
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  2. R. Boopathy
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Correspondence to T. Palvannan.

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Palvannan, T., Boopathy, R. Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media. World J Microbiol Biotechnol 21, 1153–1155 (2005). https://doi.org/10.1007/s11274-005-0299-z

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  • DOI: https://doi.org/10.1007/s11274-005-0299-z

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