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Newly Isolated Lipolytic and Oleaginous Fungal Strain, Production, Optimization and Biochemical Characterization of the Extracellular (phospho)lipase

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Abstract

The present study investigates the biochemical characterization of an extracellular (phospho)lipase from a wood fungus Peziza sp. (medium optimization, inducer concentration and substrate specificity measurements). The strain was identified on the basis of ITS1/ITS4 primers. A 604 bp fragment was amplified by PCR and the obtained nucleotide sequence, showed 99% identity with the ITS region of isolates named as Peziza sp. Interestingly, Peziza sp. has both lipase and phospholipase activities with the same level which require both the presence of Ca2+ and bile salts. Our result shows that the lipase hydrolyzes preferably the olive oil at 45 °C, pH 8. Whereas, the phospholipase activity was detected on pure PC at 45 °C, pH 9. Lipid extraction from dry biomass using chloroform/methanol (2/1) and quantitative measurement using electron microscope showed that intracellular triglycerides content was significantly high and reaches 20.88%. Gas chromatography analysis shows a majority of C18:1 (76, 98%) and C18:2 (9, 33%). Whereas, saturated fatty acids ranging C16–C20 represent only 11.5% of total lipids composition.

Graphic Abstract

Lipid accumulation test in Pezizales sp under fluorescence microscope

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Abbreviations

CPG:

Gas chromatography analysis

PDA:

Potato Dextrose Agar

PCR:

Polymerisation Chain Reaction

PC:

Phosphatidyl choline

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Acknowledgements

This work is a part of a doctoral thesis by Ameni KTATA. Whose research was supported financially by “Ministère de l’enseignement supérieur, de la recherche scientifique et de la technologie-Tunisia” through a grant to “Laboratoire de Biochimie et Génie Enzymatique des Lipases-ENIS”.

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  1. Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax, ENIS Route de Soukra, BP 1173, 3038, Sfax, Tunisie

    Ameni Ktata, Sofiane Bezzine, Adel Sayari & Aida Karray

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  1. Ameni Ktata
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  2. Sofiane Bezzine
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Correspondence to Aida Karray.

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Ktata, A., Bezzine, S., Sayari, A. et al. Newly Isolated Lipolytic and Oleaginous Fungal Strain, Production, Optimization and Biochemical Characterization of the Extracellular (phospho)lipase. Waste Biomass Valor 11, 6677–6687 (2020). https://doi.org/10.1007/s12649-019-00907-3

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