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Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha

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Abstract

Biosynthesis of PHAs by Raltonia eutropha during the dual nutrient-limitation-zone was investigated with mixed organic acids as carbon sources and (NH4)2SO4 as nitrogen source. Two different methods of maintaining the dual-nutrient-limitation zone were adopted by feeding mixed acids and (NH4)2SO4 at determined rates into the fermentation cultures which were initially free of carbon sources (method A) or nitrogen sources (method B). The results indicate that, firstly, with the increase of the width of the dual-nutrient-limitation zone, the yield of short-chain-length-polyhydroxyalkanoates also increases and it suggests that most of the short-chain-length-polyhydroxyalkanoates were biosynthesized during the dual-nutrient-limitation zone. Secondly, in contrast with the dual-nutrient-limitation method of limiting the nitrogen source first (method B), the dual-nutrient-limitation method of limiting the carbon source first (method A) was more favourable for the production of short-chain-length-polyhydroxyalkanoates, and the maximum production of short-chain-length-polyhydroxyalkanoates of these two methods are 3.72 and 2.55 g/l, respectively.

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Authors and Affiliations

  1. Open Laboratory of Chirotechnology of the Institute of Molecular for Drug Discovery and Synthesis, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Hum, Kowloon, Hong Kong

    Qun Yan, Ying Sun, Lifang Ruan & Peter Hoi Fu Yu

  2. Ministry of Education, Key Laboratory of Industrial Biotechnology, Southern Yangtze University, Wuxi, 214036, China

    Jian Chen

Authors
  1. Qun Yan
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  2. Ying Sun
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  3. Lifang Ruan
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  4. Jian Chen
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  5. Peter Hoi Fu Yu
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Correspondence to Peter Hoi Fu Yu.

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Yan, Q., Sun, Y., Ruan, L. et al. Biosynthesis of short-chain-length-polyhydroxyalkanoates during the dual-nutrient-limited zone by Ralstonia eutropha. World J Microbiol Biotechnol 21, 17–21 (2005). https://doi.org/10.1007/s11274-004-0877-5

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  • DOI: https://doi.org/10.1007/s11274-004-0877-5

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