Abstract
Bioremediation processes have been credited for reducing high levels of organic contaminants from soils. However, during the bioremediation of soils contaminated with diesel, the conversion of heavy molecules to volatile organic compounds (VOCs) and greenhouse gases (GHGs) and the volatilization of light molecules can occur. The ongoing construction of a large petrochemical complex in Rio de Janeiro (COMPERJ) and the transportation of large volumes of oil by-products have raised serious concerns regarding accidents that may result in soil contamination. Bioremediation is a potential technique that can be applied to minimize damage from such contamination. The objective of this study was to characterize the emission of GHGs and VOCs during the bioremediation of soils contaminated with diesel oil. Soil samples contaminated with 0.5, 2.0, and 4.0 w/w% diesel oil were kept in glass rectors (2 L internal volume) for 3 months under anaerobic/anoxic conditions. The soil moisture was kept at 80 % of the field capacity. Bioremediation processes were investigated in regard to nutrient adjustment (biostimulation), no adjustment (natural attenuation), and sterilized soil (abiotic process). The gases emitted from various reactors were collected with coconut shell charcoal cartridges, and the GHGs were collected in Tedlar bags. The chemical analyses of GHGs and VOCs were performed using gas chromatography. The results indicated that air samples contained high concentrations of CO2, but low concentrations of CH4. Differences in the composition of the gas emitted, regarding CO2, were not statistically significant. Regarding VOC emissions, such as alkanes and alkenes (both branched), cycloalkanes, and aromatic-substituted compounds, the compounds with higher emissions were cycloalkanes and branched alkanes.
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Ausma, S., Edwards, G. C., Fitzgerald-hubble, C. R., Halfpenne, L. M., Gillespie, T. J., & Mortimer, W. P. (2002). Volatile hydrocarbon emissions from a diesel fuel contaminated soil bioremediation facility. Journal of the Air and Waste Management Association, 52(7), 769–780.
Barret, M., Carrière, H., Delgadillo, K., & Patureau, D. (2010). PAH fate during the anaerobic digestion of contaminated sludge: do bioavailability and/or cometabolism limit their biodegradation? Water Research, 44(13), 3797–3806.
Bartha, M. R. (1981). Problems associated with the use of azide as an inhibitor of microbial activity in soil. Applied and Environmental Microbiology, 41(3), 833–836.
Bertrand, A.R. (1965). Rate of water intake in the field. In: BLACK, C.A., ed. Methods of soil analysis. American Society of Agronomy, 1, 197–209.
Bohn, H. L., Mcneal, B. L., & O'connor, G. A. (1979). Soil Chemistry. New York: Wiley.
Brener, C. P., & Jackson, M. C. (1970). Mineralogical analysis of clays in soils developed from basalts in Australia. Israel Journal of Chemistry, 8, 481–500.
Chiriac, R., De Araújo Morais, J., Carre, J., Bayard, R., Chovelan, J. M., & Gourdon, R. (2011). Study of the VOC emission from a municipal solid waste storage pilot-scale cell: comparison with biogases from municipal waste landfill site. Waste Management, 31(11), 2294–2301.
Colla, T. S., Andreazza, R., Bucker, F., Souza, M. M., Tramontini, L., Prado, G. R., et al. (2013). Bioremediation assessment of diesel–biodiesel-contaminated soil using an alternative bioaugmentation strategy. Environmental Science and Pollution Research. doi:10.1007/s11356-013-2139-2.
Da Cruz, G. F., Vasconcellos, S. P., Angolini, C. F. F., Dellagnezze, B. M., Garcia, I. N. S., Oliveira, V. M., et al. (2011). Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microorganisms in deed sea reservoirs? AMB Express, 1, 45–47.
Díaz, E. (2004). Bacterial degradation of aromatic pollutants: a paradigm of metabolic versatility. International Microbiology, 7, 173–180.
Diplock, E. E. (2009). Predicting bioremediation of hydrocarbons: laboratory to field scale. Environmental Pollution, 157(6), 1831–1840.
Eibes, G., Cajthmal, T., Moreira, M. T., Feijo, G., & Lema, J. M. (2006). Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone. Chemosphere, 64(3), 408–414.
EMBRAPA. (1997). Centro Nacional de Pesquisa de Solos (Rio de Janeiro, RJ). Manual de métodos de análise de solos (in Portuguese). 2. ed. — Rio de Janeiro: EMBRAPA — CNPS, 212 p.
Gan, S., Lau, E. V., & Ng, H. K. (2009). Remediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). Journal of Hazardous Materials, 172(2–3), 532–549.
Giostra, U., Furlani, F., Arduini, J., Cava, D., Manning, A. J., O’Doherty, J. J., et al. (2011). The determination of a “regional” atmospheric background mixing ratio for anthropogenic greenhouse gases: a comparison of two independent methods. Atmospheric Environment, 45(39), 7396–7405.
Hafner, S. D., Howard, C., Muck, R. E., Franco, R. B., Montes, F., Green, P. G., et al. (2013). Emission of volatile organic compounds from silage: compounds, sources and implications. Atmospheric Environment, 77, 828–839.
Haritash, A. K., & Kaushik, C. P. (2009). Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. Journal of Hazardous Materials, 169(1–3), 1–15.
He, X., Lau, A. K., Sokhansanj, S., Lim, C. J., Bi, X. T., & Melin, S. (2012). Dry master losses in combination with gaseous emissions during the storage of forest residues. Fuel, 95, 662–664.
Iranzo, M., Sainz-Padro, I., Boluda, R., Sanchez, J., & Mormeneo, S. (2001). The use of microorganisms in environmental engineering. Annals of Microbiology, 51, 135–143.
Jacques, R., & Seminoti, J. (2006). Biorremediação de solos contaminados com hidrocarbonetos aromáticos policíclicos. São Gabriel: UNIPAMPA.
Jϕrgensen, K. S. (2011). In situ bioremediation. Reference module in earth systems an environmental sciences — Comprehensive Biotechnology, 2nd ed. 59–67.
Karamallidis, A. K., Evangelou, A. C., Karabika, E., Kaikkou, A. I., Drainas, C., & Voudrias, E. A. (2010). Laboratory scale of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain Spet. Bioresource Technology, 101(16), 6545–6552.
Koornenerf, J., Ramírez, A., Turkenburg, W., & Faaj, A. (2012). The environment impact and risk assessment of CO2 capture, transport and storage — an evaluation of the knowledge base. Progress in Energy and Combustion Science, 38(1), 62–86.
Li, X. Z., Lin, X. G., Zhang, J., Wu, Y. C., Yin, R., Feng, Y. Z., et al. (2010). Degradation of polycyclic aromatic hydrocarbons by crude extracts from spent mushroom substrate and its possible mechanisms. Current Microbiology, 60(5), 336–342.
Liebeg, E. W., & Cutright, T. J. (1999). The investigation of enhanced bioremediation through the addition of macro and micro nutrients in a PAH contaminated soil. International Biodeterioration & Biodegradation, 44, 55–64.
Milić, J. S., Beškoski, V. P., Ilić, M. V., Ali, S. A. M., Gojgić-Cvijović, G. Đ., & Vrvić, M. M. (2009). Bioremediation of soil heavily contaminated with crude oil and its products: composition of the microbial consortium. Journal of the Serbian Chemical Society, 74(4), 455–460.
Militon, C., Boucher, D., Vachelard, C., Perchet, G., Barra, V., Troquet, J., et al. (2010). Bacterial community changes during bioremediation of aliphatic hydrocarbon-contaminated soil. FEMS Microbiology Ecology, 74(3), 669–681.
Mumford, K. A., Dayner, J. K., Snape, I., Starck, S. C., Stevens, G. W., & Gore, D. B. (2013). Design installation and preliminary testing of permeable reactive barrier for diesel fuel remediation at Casey Station, Antarctica. Cold Region Science and Technology, 96, 96–107.
Nakagawa, L. E., & Andréa, M. M. (2006). Efeito de alterações nas características do solo sobre a degradação de hexaclorobenzeno. Revista Brasileira de Ciência do Solo (In Portuguese), 30(3), 575–582.
Nester, E. W., Anderson, D. G., Roberts, C. E., Jr., Pearsall, N. N., & Nester, M. T. (2001). Microbiology: a human perspective (3rd ed.). New York: McGraw-Hill.
NIOSH. Manual of analytical methods. (2003). Fourth edition, hydrocarbons — Method 1500, Issue 3.
Palmieri, F., Santos, H. G., Gomes, I. A., Lumbreras, J. F., & Aglio, M. L. D. (2003). The Brazilian soil classification system. In H. Eswaran, T. Rice, R. Ahrens, & B. A. Stewart (Eds.), Soil classification: A global desk reference (pp. 127–146). Boca Raton: CRC Press.
Pasumarthi, R., Cahndrasekaran, S., & Mutnuri, S. (2013). Biodegradation of crude oil by Pseudomonas aeruginosa and Escherichia fergusonii isolated from the Goan Coast. Marine Pollution Bulletin, 76(1–2), 276–282.
Perfumo, A., Ibrahim, M., Roger, M., & Luigi, V. (2007). Thermally enhanced approaches for bioremediation of hydrocarbon-contaminated soils. Chemosphere, 66, 179–184.
Peters, K. E., Walters, C. C., & Moldowan, J. M. (2005). The biomarker guide: Biomarkers and isotopes in the environment and human history (2nd ed.). Cambridge University Press: United Kingdom.
Rodrigues, A., Nogueira, R., Melo, L. F., & Brito, A. G. (2013). Effect of low concentrations of synthetic surfactants on polycyclic aromatic hydrocarbons biodegradation. International Biodeterioration & Biodegradation, 83, 48–55.
Sarkar, D., Ferguson, M., Data, R., & Birnbaum, S. (2005). Bioremediation of petroleum hydrocarbons in contaminated soils: comparison of biosolids addition, carbon supplementation, and monitored natural attenuation. Environmental Pollution, 136, 187–195.
Singh, A., & Ward, O. P. (2004). Applied bioremediation and phytoremediation (soil biology — book 1). New York: Springer. 281 p.
Solomons, T. W. G., & Fryhle, C. B. (2011). Organic chemistry. New York: John Wiley & Sons. 744 p.
Tammadoni, M., Sotudeh-Gharebagh, R., Nario, S., Hajihosseinzadeh, M., Mostoufi, N. (2013). Experimental study of the VOC emitted from crude oil tankers. Process Safety and Environmental Protection. In press, corrected proof.
Trevors, J. T. (1996). Sterilization and inhibition of microbial activity in soil. Journal of Microbiological Methods, 26(1–2), 53–59.
U.S. EPA. (1984). Method TO-2. Method for the determination of volatile organic compounds in ambient air by carbon molecular sieve adsorption and gas chromatography/mass spectrometry (GC/MS). Revision 1.0.
U.S. EPA. (1996). A citizen’s guide to bioremediation. EPA 542-F-96-007, 1–4.
Wang, X. D., Zhou, S. M., & Wang, A. L. (2005). Biodegradation of imazapyr in typical soils in Zhejiang Province, China. Journal of Environmental Sciences, 17(4), 593–597.
Yu, S. H., Ke, L., Wong, Y. S., & Tam, N. F. Y. (2005). Degradation of polycyclic aromatic hydrocarbons (PAHS) by a bacterial consortium enriched from mangrove sediments. Environmental International, 31(2), 149–154.
Zou, S. C., Lee, S. C., Chan, C. Y., Ho, K. F., Wang, X. M., & Chan, L. Y. (2003). Characterization of ambient volatile organic compounds at a landfill site in Guangzhou, South China. Chemosphere, 51, 1015–1022.
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The financial support from the Rio de Janeiro Foundation for Research Assistance (FAPERJ) as well as the Brazilian National Council for Scientific and Technological Development (CNPq) is acknowledged. The support for international exchange from the Swedish Foundation of International Cooperation in Research and Higher Education (STINT) was also appreciated.
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Franco, M.G., Corrêa, S.M., Marques, M. et al. Emission of Volatile Organic Compounds and Greenhouse Gases from the Anaerobic Bioremediation of Soils Contaminated with Diesel. Water Air Soil Pollut 225, 1879 (2014). https://doi.org/10.1007/s11270-014-1879-z
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DOI: https://doi.org/10.1007/s11270-014-1879-z