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Degradation of Polycyclic Aromatic Hydrocarbons by Crude Extracts from Spent Mushroom Substrate and its Possible Mechanisms

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Abstract

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by pure laccase has been reported, but the high cost limited its application in environmental bioremediation. Here, we reported a study about PAHs degradation by crude extracts (CEs) containing laccase, which were obtained by extracting four spent mushroom (Agaricus bisporus, Pleurotus eryngii, Pleurotus ostreatus, and Coprinus comatus) substrates. The results showed that anthracene, benzo[a]pyrene, and benzo[a]anthracene were top three degradable PAHs by CEs while naphthalene was most recalcitrant. The PAHs oxidation was enhanced in the presence of 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Laccase included in CE might play a major role in PAHs degradation. The maximum degradation rate of anthracene and benzo[a]pyrene was observed by using crude extracts from P. eryngii while the highest laccase activities were found in crude extracts from A. bisporus, moreover, crude extracts from P. eryngii, which contained less laccase activities, degraded more anthracene and benzo[a]pyrene than pure laccase with higher laccase activities. The lack of correlation between laccase activity and PAHs degradation rate indicated that other factors might also influence the PAHs degradation. Boiled CEs were added to determine the effect on PAHs degradation by laccase. The results showed that all four boiled CEs had improved the PAHs oxidation. The maximum improvement was observed by adding CEs from P. eryngii. It suggested that some mediators indeed existed in CEs and CEs from P. eryngii contained most. As a result, CEs from P. eryngii has the most application potential in PAHs bioremediation.

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Acknowledgment

This study was supported by the Ministry of Science and Technology of the People’s Republic of China (2007AA061101) and National Natural Science Foundation of China (40801091); WU Y-C was partly supported by a IFS grant (C/4471-1).

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Authors and Affiliations

  1. State Key Laboratory of Soil and Sustainable Agriculture, CAS Key Laboratory of Soil Environment and Pollution Remediation, Joint Open Laboratory of Soil and the Environment, Hongkong Baptist University & Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Xuanzhen Li, Xiangui Lin, Jing Zhang, Yucheng Wu, Rui Yin, Youzhi Feng & Yong Wang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Xuanzhen Li

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  1. Xuanzhen Li
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  2. Xiangui Lin
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Correspondence to Xiangui Lin.

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Li, X., Lin, X., Zhang, J. et al. Degradation of Polycyclic Aromatic Hydrocarbons by Crude Extracts from Spent Mushroom Substrate and its Possible Mechanisms. Curr Microbiol 60, 336–342 (2010). https://doi.org/10.1007/s00284-009-9546-0

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  • DOI: https://doi.org/10.1007/s00284-009-9546-0

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