Skip to main content
SpringerLink
Log in

Characterization of Heavy Metal-Resistant Endophytic Yeast Cryptococcus sp. CBSB78 from Rapes (Brassica chinensis) and Its Potential in Promoting the Growth of Brassica spp. in Metal-Contaminated Soils

  • Published:
Water, Air, & Soil Pollution Aims and scope Submit manuscript

Abstract

A Cd-, Pb-, Zn-, Cu-resistant endophytic yeast CBSB78 was isolated from surface-sterilized rape roots. The isolate was identified as Cryptococcus sp. based on the ITS1–5.8S–ITS2 sequence analysis. The strain was resistant to 20 mM Cd2+, 20 mM Pb2+, 10 mM Zn2+, and 7 mM Cu2+. The yeast CBSB78 was a low indole-3-acetic acid (IAA) producer and possessed low 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Overall, 29.4–244 % of survival rates increased and the dry weight of Brassica alboglabra showed a 41.1 % increase when it was inoculated into the seedlings. The inoculation of CBSB78 could also increase the extraction amounts of Cd, Pb, and Zn by B. alboglabra simultaneously in the multi-metal contaminated soils, which showed the potential to improve extraction efficacy of Cd, Pb, Zn by B. alboglabra seedlings in the field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Bouaid, A., Martinez, M., & Aracil, J. (2009). Production of biodiesel from bioethanol and Brassica carinata oil: oxidation stability study. Bioresource Technology, 100, 2234–2239.

    Article  CAS  Google Scholar 

  • Cao, L., Jiang, M., Zeng, Z., Du, A., Tan, H., & Liu, Y. (2008). Trichoderma atroviride F6 improves phytoextraction efficiency of mustard (Brassia juncea (L) Coss. var. foliosa Bailey) in Cd, Ni contaminated soils. Chemosphere, 71, 1769–1773.

    Article  CAS  Google Scholar 

  • Cardone, M., Mazzoncini, M., Menini, S., Rocco, V., Senatore, A., Seggiani, M., et al. (2003). Brassica carinata as an alternative oil crop for the production of biodiesel in Italy: agronomic evaluation, fuel production by transesterification and characterization. Biomass and Bioenergy, 25, 623–636.

    Article  CAS  Google Scholar 

  • Cho, D. H., & Kim, E. Y. (2003). Characterization of Pb2+ biosorption from aqueous solution by Rhodotorula glutinis. Bioprocess and Biosystems Engineering, 25, 271–277.

    CAS  Google Scholar 

  • Cloete, K. J., Valentine, A. J., Stander, M. A., Blomerus, L. M., & Botha, A. (2009). Evidence of symbiosis between the soil yeast Crytococcus laurentii and a sclerophyllous medicinal shrub, Agathosma betulina (Berg.) Pillans. Microbial Ecology, 57, 624–632.

    Article  Google Scholar 

  • D’Annibale, A., Rosetto, F., Leonardi, V., Federici, F., & Petruccioli, M. (2006). Role of autochthonous filamentous fungi in bioremediation of a soil historically contaminated with aromatic hydrocarbons. Applied and Environmental Microbiology, 72, 28–36.

    Article  Google Scholar 

  • Deng, Z., Cao, L., Huang, H., Jiang, X., Wang, W., Shi, Y., et al. (2011). Characterization of Cd- and Pb-resistant fungal endophyte Mucor sp. CBRF59 isolated from rapes (Brassica chinensis) in metal contaminated soils. Journal of Hazardous Materials, 185, 717–724.

    Article  CAS  Google Scholar 

  • Doty, S. L. (2008). Enhancing phytoremediation through the use of transgenics and endophytes. New Phytologist, 179, 318–333.

    Article  CAS  Google Scholar 

  • El-Tarabily, K. A. (2008). Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic acid deaminases- producing streptomycete actinomycetes. Plant and Soil, 308, 161–174.

    Article  CAS  Google Scholar 

  • El-Tarabily, K. A., & Sivasithamparam, K. (2006). Potential of yeasts as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Mycoscience, 47, 25–35.

    Article  Google Scholar 

  • Hontzeas, N., Richardson, A. O., Belimov, A., Safronova, V., Abu-Omar, M. M., & Glick, B. R. (2005). Evidence for horizontal transfer of 1-aminocyclopropane-1-carboxylate deaminase genes. Applied and Environmental Microbiology, 71, 7556–7558.

    Article  CAS  Google Scholar 

  • Isaeva, O. V., Glushakova, A. M., Garbuz, S. A., Kachalkin, A. V., & Chernov, I. Y. (2010). Endophytic yeast fungi in plant storage tissues. Biology Bulletin, 37, 26–34.

    Article  CAS  Google Scholar 

  • Jiang, M., Cao, L. X., & Zhang, R. D. (2008). Effects of Acacia (Acacia auriculaeformis A. Cunn)-associated fungi on mustard (Brassica juncea (L.) Coss. var. foliosa Bailey) growth in Cd- and Ni-contaminated soils. Letters in Applied Microbiology, 47, 561–565.

    Article  CAS  Google Scholar 

  • Ksheminska, H., Jaglarz, A., Fedorovych, D., Babyak, L., Yanovych, D., Kaszycki, P., et al. (2003). Bioremediation of chromium by the yeast Pichia guilliermondii: toxicity and accumulation of Cr (III) and Cr (VI) and the influence of riboflavin on Cr tolerance. Microbiological Research, 158, 59–67.

    Article  CAS  Google Scholar 

  • Kuiper, I., Lagendijk, E. L., Bloemberg, G. V., & Lugtenberg, B. J. J. (2004). Rhizoremediation: a beneficial plant–microbe interaction. Molecular Plant-Microbe Interactions, 17, 6–15.

    Article  CAS  Google Scholar 

  • Lai, X., Cao, L., Tan, H., Fang, S., Huang, Y., & Zhou, S. (2007). Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China sea. The ISME Journal, 1, 756–762.

    Article  CAS  Google Scholar 

  • Lebeau, T., Braud, A., & Jézéquel, K. (2008). Performance of bioaugmentation-assisted phytoextraction applied to metal contaminated soils: a review. Environmental Pollution, 153, 497–522.

    Article  CAS  Google Scholar 

  • Mehra, R. K., & Winge, D. R. (2004). Metal ion resistance in fungi: molecular mechanisms and their regulated expression. Journal of Cellular Biochemistry, 45, 30–40.

    Article  Google Scholar 

  • Nassar, A. H., El-Tarabily, K. A., & Sivasithamparam, K. (2005). Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots. Biology and Fertility of Soils, 42, 97–108.

    Article  CAS  Google Scholar 

  • Newman, L. A., & Reynolds, C. M. (2005). Bacteria and phytoremediation: new uses for endophytic bacteria in plants. Trends in Biotechnology, 23, 6–8.

    Article  CAS  Google Scholar 

  • Perego, P., & Howell, S. B. (1997). Molecular mechanisms controlling sensitivity to toxic metal ions in yeast. Toxicology and Applied Pharmacology, 147, 312–318.

    Article  CAS  Google Scholar 

  • Rajkumar, M., Ae, N., & Freitas, H. (2009). Endophytic bacteria and their potential to enhance heavy metal phytoextraction. Chemosphere, 77, 153–160.

    Article  CAS  Google Scholar 

  • Rosewarne, C. P., Pettigrove, V., Stokes, H. W., & Parsons, Y. M. (2010). Class1integrons in benthic bacterial communities: abundance, association withTn402-like transposition modules and evidence forcoselection with heavy-metal resistance. FEMS Microbiology Ecology, 72, 35–46.

    Article  CAS  Google Scholar 

  • Ryan, R., Germaine, K., Franks, A., Ryan, D. J., & Dowling, D. N. (2008). Bacterial endophytes; recent developments and applications. FEMS Microbiology Letters, 278, 1–9.

    Article  CAS  Google Scholar 

  • Segura, A., Rodríguez-Conde, S., Ramos, C., & Ramos, J. L. (2009). Bacterial responses and interactions with plants during rhizoremediation. Microbial Biotechnology, 2, 452–464.

    Article  CAS  Google Scholar 

  • Sessitsch, A., & Puschenreiter, M. (2008). Endophytes and rhizosphere bacteria of plants growing in heavy metal-containing soils. In P. Dion & C. S. Nautiyal (Eds.), Microbiology of extreme soils (pp. 317–332). Germany: Springer-Verlag.

    Chapter  Google Scholar 

  • Sheng, X. F., Xia, J. J., Jiang, L. Y., He, L. Y., & Qian, M. (2008). Characterization of heavy metal-resistant endophytic bacteria from rape (Brassica napus) roots and their potential in promoting the growth and lead accumulation of rape. Environmental Pollution, 156, 1164–1170.

    Article  CAS  Google Scholar 

  • Tani, A., Zhang, D., Duine, J. A., & Kawai, F. (2004). Treatment of the yeast Rhodotorula glutinis with AlCl3 leads to adaptive acquirement of heritable aluminum resistance. Applied Microbiology and Biotechnology, 65, 344–348.

    Article  CAS  Google Scholar 

  • Usuki, F., & Narisawa, K. (2007). A mutualistic symbiosis between a dark septate endophytic fungus, Heteroconium chaetospira, and a nonmycorrhizal plant, Chinese cabbage. Mycologia, 99, 175–184.

    Article  CAS  Google Scholar 

  • Vadkertiová, R., & Sláviková, E. (2006). Metal tolerance of yeasts isolated from water, soil and plant environment. Journal of Basic Microbiology, 46, 145–152.

    Article  Google Scholar 

  • Wenzel, W. W. (2009). Rhizophere processes and management in plant-assisted bioremediation (phytoremediation) of soils. Plant and Soil, 321, 385–408.

    Article  CAS  Google Scholar 

  • Weyens, N., Croes, S., Dupae, J., Newman, L., van der Lelie, D., Carleer, R., et al. (2010). Endophytic bacteria improve phytoremediation of Ni and TCE co-contamination. Environmental Pollution, 158, 2422–2427.

    Article  CAS  Google Scholar 

  • Wright, M. S., Peltier, G. L., Stepanauskas, R., & McArthur, J. V. (2006). Bacterial tolerances to metal and antibiotics in metal-contaminated and reference streams. FEMS Microbiology Ecology, 58, 293–302.

    Article  CAS  Google Scholar 

  • Xin, G., Glawe, D., & Doty, S. L. (2009). Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees. Mycological Research, 113, 973–980.

    Article  CAS  Google Scholar 

  • You, J. L., Cao, L. X., Liu, G. F., Zhou, S. N., Tan, H. M., & Lin, Y. C. (2005). Isolation and characterization of actinomycetes antagonistic to pathogenic Vibrio spp. from nearshore marine sediments. World Journal of Microbiology and Biotechnology, 21, 679–682.

    Article  Google Scholar 

Download references

Acknowledgements

This work was partly supported by grants from the Chinese National Natural Science Foundation (Nos. 51039007 and 51179212), the Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology (2011K0004), and the Fundamental Research Funds for the Central Universities.

Author information

Authors and Affiliations

  1. School of Basic courses, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Zujun Deng

  2. School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    Wenfeng Wang, Hongming Tan & Lixiang Cao

Authors
  1. Zujun Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenfeng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hongming Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lixiang Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lixiang Cao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Deng, Z., Wang, W., Tan, H. et al. Characterization of Heavy Metal-Resistant Endophytic Yeast Cryptococcus sp. CBSB78 from Rapes (Brassica chinensis) and Its Potential in Promoting the Growth of Brassica spp. in Metal-Contaminated Soils. Water Air Soil Pollut 223, 5321–5329 (2012). https://doi.org/10.1007/s11270-012-1282-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11270-012-1282-6

Keywords

Search

Navigation