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Breakdown Products in the Metabolic Pathway of Anthracene Degradation by a Ligninolytic Fungus Polyporus sp. S133

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Abstract

Polyporus sp. S133 fungi were selected based on their ability to degrade anthracene in liquid media. The degradation efficiency of anthracene increased by adding 0.5% Tween 80 to reach 71%; agitation at 120 rev/min increased the degradation to 92% after 30 days of incubation. Enzymes such as manganese peroxidase (MnP), lignin peroxidase (LiP), laccase, 1,2-dioxygenase and 2,3-dioxgenase were produced by Polyporus sp. S133 during incubation, and the highest enzyme activity was 182.3 U l−1 by 1,2-dioxygenase after 20 days of incubation. These results indicate that ligninolytic and dioxygenase enzymes secreted from Polyporus sp. S133 could play an important role in anthracene degradation efficiency. The metabolites detected through the degradation pathway were anthraquinone, phthalic acid, benzoic acid and catechol.

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Acknowledgements

A part of this research was financially supported by Research University Grant of Universiti Technologi Malaysia (vote no. QJ13000.7122.00 J31), which is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Environmental and Water Research Management, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Tony Hadibarata, Ameer Badr Khudhair & Mohd Razman Salim

  2. Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Ameer Badr Khudhair

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  1. Tony Hadibarata
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  2. Ameer Badr Khudhair
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  3. Mohd Razman Salim
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Correspondence to Ameer Badr Khudhair.

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Hadibarata, T., Khudhair, A.B. & Salim, M.R. Breakdown Products in the Metabolic Pathway of Anthracene Degradation by a Ligninolytic Fungus Polyporus sp. S133. Water Air Soil Pollut 223, 2201–2208 (2012). https://doi.org/10.1007/s11270-011-1016-1

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