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The Fate of Cryptosporidium Parvum Oocysts in Reclaimed Water Irrigation-history and Non-history Soils Irrigated with Various Effluent Qualities

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Abstract

The present study aimed to look at the fate of protozoan parasite Cryptosporidium parvum oocysts applied through surface drip irrigation on reclaimed water irrigation-history and non-history sandy-loam (Hamra) soil columns. A new and simple isolation method for recovery of oocysts from soil samples was developed and used along this study. The new soil isolation method of oocysts is based on the “two phase separation method” formerly used to recover Clostridium perfringens spores from sediments and soil samples with minor modifications. The range recovery achieved by this method was 64–95% (mean 61.2 ± 17.4). The objectives of the second part of this study were to investigate several physical and chemical factors governing transport and survival of C. parvum oocysts in sandy-loam soil columns by breakthrough curves. Comparison of fresh water and reclaimed water irrigation revealed that reclaimed water irrigated-history soil was more hydrophobic allowing water flow through channels with poor oocysts retention and fast flow. Examination of the organic matter effect (originating from reclaimed water irrigation) on oocysts breakthrough revealed that their soil infiltration increased. Calculations of oocysts concentration at different columns depths showed that most of the oocysts were retained in the first 5 cm of soil column. In the present study, comparing the two soil types (history and non-history of effluents irrigation) beside the surface electrostatic charge, one of the main elements found to affect oocysts infiltration and transport in soil columns was soil hydrophobicity caused by soluble organic matter originating from reclaimed water irrigation. Therefore, prior to application in soil irrigation, reclaimed water should be treated to high quality (i.e. membrane technology as the best option) to prevent enhanced transport of various pathogens through those irrigated soils.

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References

  • Armon, R., Gold, D., Brodsky, M., & Oron, G. (2002). Surface and subsurface irrigation with effluents of different qualities and presence of Cryptosporidium oocysts in soil and on crops. Water Science and Technology, 46(3), 115–122.

    CAS  Google Scholar 

  • Atwill, E. R., Hou, L., Karle, B. M., Harter, T., Tate, K. W., & Dahlgren, R. A. (2002). Transport of Cryptosporidium parvum oocysts through vegetated buffer strips and estimated filtration efficiency. Applied and Environmental Microbiology, 68(11), 5517–5527.

    Article  CAS  Google Scholar 

  • Bradford, S. A., & Bettahar, M. (2005). Straining, attachment, and detachment of Cryptosporidium oocysts in saturated porous media. Journal of Environmental Quality, 34(2), 469–478.

    Article  CAS  Google Scholar 

  • Brush, C. F., Walter, M. F., Anguish, L. J., & Ghiorse, W. C. (1998). Influence of pretreatment and experimental conditions on electrophoretic mobility and hydrophobicity of Cryptosporidium parvum oocysts. Applied and Environmental Microbiology, 64(11), 4439–4445.

    CAS  Google Scholar 

  • Bukhari, Z., Smith, H. V., Sykes, N., Humphreys, S. W., Paton, C. A., Girdwood, R. W. A., et al. (1997). Occurrence of Cryptosporidium spp oocysts and Giardia spp cysts in sewage effluents and effluents from treatment plants in England. Water Science and Technology, 35(11–12), 385–390.

    Article  CAS  Google Scholar 

  • Bukhari, Z., Glen, W. G., Clancy, J. L. (1999) Effects of pH on a fluorogenic vital dyes assay (4′,6′-diamidino-2-phenyl-indole and propidium iodide) for Cryptosporidium sp. oocysts. Water Research, 33(13), 3037–3039.

    Article  CAS  Google Scholar 

  • Butkus, M. A., Bays, J. T., & Labare, M. P. (2003). Influence of surface characteristics on the stability of Cryptosporidium parvum oocysts. Applied and Environmental Microbiology, 69(7), 3819–3825.

    Article  CAS  Google Scholar 

  • Considine, R. F., Dixon, D. R., Drummond, C. J. (2002). Oocysts of Cryptosporidium parvum and model sand surfaces in aqueous solutions: An Atomic Force Microscope (AFM) study. Water Research, 36(14), 3421–3428.

    Article  CAS  Google Scholar 

  • Campbell, A. T., Robertson, L. J., Smith, H. V. (1992).Viability of Cryptosporidium parvum oocysts: Correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Applied and Environmental Microbiology, 58(11), 3488–3493.

    CAS  Google Scholar 

  • Dai, X., & Boll, J. (2003). Evaluation of attachment of Cryptosporidium parvum and Giardia lamblia to soil particles. Journal of Environmental Quality, 32, 296–304.

    Article  CAS  Google Scholar 

  • Drozd, C., & Schwartzbrod, J. (1996). Hydrophobic and electrostatic cell surface properties of Cryptosporidium parvum. Applied and Environmental Microbiology, 62(4), 1227–1232.

    CAS  Google Scholar 

  • Emerson, D. J., & Cabelli, V. J. (1982). Extraction of Clostridium perfringens spores from bottom sediment samples. Applied and Environmental Microbiology, 44(5), 1144–1149.

    CAS  Google Scholar 

  • Gennaccaro, A. L., McLaughlin, M. R., Quintero-Betancourt, W., Huffman D. E., & Rose, J. B. (2003). Infectious Cryptosporidium parvum oocysts in final reclaimed effluent. Applied and Environmental Microbiology, 69(8), 4983–4984.

    Article  CAS  Google Scholar 

  • Harter, T., Wagner, S., & Atwill, E. R. (2000). Colloid transport and filtration of Cryptosporidium parvum in sandy soil and aquifer sediments. Environmental Science & Technology, 34, 62–70.

    Article  CAS  Google Scholar 

  • Kuznar, Z. A., & Elimelech, M. (2005). Role of surface proteins in the deposition kinetics of Cryptosporidium parvum oocysts. Langmuir, 21(2), 710–716.

    Article  CAS  Google Scholar 

  • Levy, G. J., Rosenthal, A., Tarchitzky, J., Shainberg, I., & Chen, Y. (1999). Soil hydraulic conductivity changes caused by irrigation with reclaimed waste water. Journal of Environmental Quality, 28(5), 1658–1664.

    Article  CAS  Google Scholar 

  • Logan, A. J., Stevik, T. K., Siegrist, R. L., & Ronn, R. M. (2001). Transport and fate of Cryptosporidium parvum oocysts in intermittent sand filters. Water Research, 35(18), 4359–4369.

    Article  CAS  Google Scholar 

  • MacKenzie, W. R., Hoxie, N. J., Proctor, M. E., Gradus, M. S., Blair, K. A., Peterson, D. E., et al. (1994). A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. New England Journal of Medicine, 331, 161–167.

    Article  CAS  Google Scholar 

  • Madore, M. S., Rose, J. B., Gerba, C. P., Arrowood, M. J., & Sterling, C. R. (1987). Occurrence of Cryptosporidium spp. oocysts in sewage effluents and selected surface waters. Journal of Parasitology, 73, 702–705.

    Article  CAS  Google Scholar 

  • Nasser, A. M., Huberman, Z., Zilberman, A., & Greenfeld, S. (2003). Die-off and retardation of Cryptosporidium spp. oocyst in loamy soil saturated with secondary effluent. Water Science and Technology, 3(4), 253–259.

    CAS  Google Scholar 

  • Parker, J. F. W., & Smith, H. V. (1991). Removal of Cryptosporidium by water treatment. Final report to the Water Research Centre. June 1991.

  • Parker, J. F. W., & Smith, H. V. (1993). Destruction of oocysts of Cryptosporidium parvum by sand and chlorine. Water Research, 27(4), 729–731.

    Article  CAS  Google Scholar 

  • Robertson, L. J., Campbell, A. T., & Smith, H. V. (1992). Survival of oocysts of Cryptosporidium parvum under various environmental pressures. Applied and Environmental Microbiology, 58, 3494–3500.

    CAS  Google Scholar 

  • Tufenkji, N., Miller, Garrett, F., Ryan, J. N., Harvey, R. W. & Elimelech, M. (2004) Transport of Cryptosporidium oocysts in porous media: role of straining and physicochemical filtration. Environmental Science and Technology, 38(22), 5932–5938.

  • Walker, M. J., & Montemagno, C. D. (1999). Sorption of Cryptosporidium parvum oocysts in aqueous solution to metal oxide and hydrophobic substrates. Environmental Science & Technology, 33(18), 3134–3139.

    Article  CAS  Google Scholar 

  • Zuckerman, U., Gold, D., Shelef, G., & Armon, R. (1997a). The presence of Giardia and Cryptosporidium in surface waters and effluents in Israel. Water Science and Technology, 35(11–12), 381–384.

    Article  CAS  Google Scholar 

  • Zuckerman, U., Gold, D., Shelef, G., Yuditsky, A., & Armon, R. (1997b). Microbial degradation of Cryptosporidium parvum by Serratia marcescens with high chitinolytic activity. In International Symposium on Waterborne Cryptosporidium Proceedings, March 2–5, pp. 297–304. Newport Beach, CA.

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Acknowledgements

The authors wish to thank Water Research Institute, Technion, Haifa for the financial support, Grant No. 015-109.

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Authors and Affiliations

  1. Faculty of Civil & Environmental, Division of Environmental, Water & Agricultural Engineering, Technion, Haifa, 32000, Israel

    K. Khashiboun, A. Zilberman & J. Starosvetsky

  2. Faculty of Civil & Environmental Engineering and Grand Water Research Institute, Division of Environmental, Water & Agricultural Engineering, Technion-IIT, Haifa, 32000, Israel

    A. Shaviv & R. Armon

Authors
  1. K. Khashiboun
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  2. A. Zilberman
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  3. A. Shaviv
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  4. J. Starosvetsky
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  5. R. Armon
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Correspondence to R. Armon.

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Khashiboun, K., Zilberman, A., Shaviv, A. et al. The Fate of Cryptosporidium Parvum Oocysts in Reclaimed Water Irrigation-history and Non-history Soils Irrigated with Various Effluent Qualities. Water Air Soil Pollut 185, 33–41 (2007). https://doi.org/10.1007/s11270-007-9420-2

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  • DOI: https://doi.org/10.1007/s11270-007-9420-2

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